Streptococcus sobrinus and Streptococcus mutans in a Longitudinal Study of Dental Caries
Streptococcus sobrinus and Streptococcus mutans in a Longitudinal Study of Dental Caries
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远缘链球菌和变异链球菌在龋齿纵向研究中的应用
DOI：
10.3402/mehd.v6i5.8142
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发表时间：
1993
影响因子：
--
通讯作者：
J. Graaff
中科院分区：
文献类型：
--
作者：
J. J. Soet;W. P. Holbrook;M. Magnúsdóttir;J. Graaff
变形链球菌与人类龋齿有关。关于变形链球菌在龋病过程中的作用，人们知道得很多，但远缘链球菌的作用还不太清楚。据报道，这种细菌在体外具有很强的产酸和耐酸能力，在大鼠身上也具有致龋性，因此，至少应该在人类身上具有一定的致龋性。本研究旨在采用非选择性培养方法和物种特异性的单抗进行纵向研究，以确定远缘链霉菌、变形链球菌与龋病的可能关系。在本研究开始时，对158名4岁儿童进行了临床检查，并对唾液中的变形链球菌进行了MSB琼脂计数。在5岁和6岁时，对儿童进行检查，并用物种特异性单抗和MSB琼脂检测变形链球菌。5-6年间，远缘链球菌的携带率从25%上升到48%。这种微生物的存在与龋患率显著相关(P=0.03)。未发现远缘链球菌的存在与唾液pH值、缓冲容量或流速之间的关系。当使用物种特异性的单抗时，远缘链球菌或变形链球菌的存在与DMFT没有显著的相关性。然而，当用选择性培养基检出变形链球菌时，这种相关性是非常显著的(P<；0.001)。这表明变形链球菌或远缘链球菌在致龋性方面存在差异。目前的纵向研究表明，远缘链霉菌与龋病发病有关，但尚不能确定其在龋病发生中的作用。关键词：变形链球菌，远缘链球菌，变形链球菌，唾液杆菌琼脂，免疫印迹，单抗，龋齿
Mutans streptococci have been correlated with human dental caries. Much is known about the role of Streptococcus mutans in the caries process, but the role of S. sobrinus is less clear. This bacterium has been reported to be very acidogenic and aciduric in vitro and cariogenic in rats and should, therefore, have at least some cariogenic potential in humans. This study was aimed at determining the possible relationship between S. sobrinus, S. mutans and dental caries in a longitudinal study by using a non-selective culturing method and species-specific monoclonal antibodies. At the beginning of this study, 158 children aged 4 yr were examined clinically and salivary mutans streptococci were counted on MSB agar. At 5 and 6 yr of age, the children were examined and mutans streptococci were detected by using species-specific monoclonal antibodies and MSB agar. The carriage rate of S. sobrinus increased between 5 and 6 yr from 25 to 48 per cent. The presence of this microorganism was significantly associated with caries incidence ( P =0.03). Relationships between the presence of S. sobrinus and salivary pH, buffer capacity or flow rate were not found. The presence of S. sobrinus or S. mutans was not significantly correlated with dmft when using the species-specific monoclonal antibodies. However, this correlation was highly significant (P< 0.001) when the mutans streptococci were detected by using the selective medium MSB. This suggests that differences in cariogenic potential occur within the species S. mutans or S. sobrinus . The present longitudinal study indicates that S. sobrinus is correlated with caries incidence, but its role in the initiation of caries could not be ascertained. Keywords - Streptococcus mutans , S. sobrinus , Mutans streptococci, Mitis salivarius agar, Immunoblotting, Monoclonal antibodies, Dental caries
饮食失调患者唾液中推定的致龋微生物水平。
影响因子：
--
作者：
Bretz,WA;Krahn,DD;Drewnowski,A;Loesche,WJ
通讯作者：
Loesche,WJ