Baculovirus expression of mouse lactoferrin receptor and tissue distribution in the mouse
Baculovirus expression of mouse lactoferrin receptor and tissue distribution in the mouse
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DOI：
10.1023/b:biom.0000027709.42733.e4
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发表时间：
2004-06-01
期刊：
影响因子：
3.5
通讯作者：
Lönnerdal, B
中科院分区：
文献类型：
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作者：
Suzuki, YA;Lönnerdal, B
乳铁蛋白（Lf）已被证明在免疫系统和小鼠胚胎的早期发育中发挥作用。Lf的特异性受体已被认为介导Lf的功能。我们最近在人胎儿小肠中发现了一种Lf受体（LfR）。因此，我们假设这种蛋白质的小鼠同源物作为LfR发挥功能。我们在杆状病毒-昆虫细胞系统中表达了小鼠Lf（MLf）和小鼠同源物（MLfR）。通过固定化重组MLf（rMLf）亲和层析纯化重组MLfR（rMLfR），证明rMLf和rMLfR之间的相互作用。RT-PCR结果显示MLfR在多种组织和胚胎发育过程中均有表达。免疫组织化学分析显示MLfR定位于各种组织，包括小肠上皮、胃、肾、卵巢和脑的各个区域。总之，MLfR作为MLf的受体发挥功能，在各种组织中表达和定位，并且可能参与MLf在早期胚胎发育期间不可或缺的功能。
Lactoferrin (Lf) has been shown to have a role in the immune system and in early development of the mouse embryo. A specific receptor for Lf has been suggested to mediate the functions of Lf. We have recently identified a Lf receptor (LfR) in human fetal small intestine. We therefore hypothesized that the mouse homologue of this protein functions as a LfR. We expressed mouse Lf (MLf) and the mouse homologue (MLfR) in a baculovirus-insect cell system. The recombinant MLfR (rMLfR) was purified by immobilized recombinant MLf (rMLf) affinity chromatography, demonstrating an interaction between rMLf and the rMLfR. RT-PCR revealed that MLfR was expressed in various tissues and during embryonic development. Immunohistochemical analysis revealed that the MLfR was localized in various tissues including small intestinal epithelium, stomach, kidney, ovary, and various regions of brain. In summary, the MLfR functions as a receptor for MLf, is expressed and localized in various tissues, and may be involved in the indispensable function of MLf during early embryonic development.