Spirochaeta smaragdinae sp. nov., a new mesophilic strictly anaerobic spirochete from an oil field
Spirochaeta smaragdinae sp. nov., a new mesophilic strictly anaerobic spirochete from an oil field
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DOI：
10.1111/j.1574-6968.1997.tb13876.x
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发表时间：
1997-10-15
影响因子：
2.1
通讯作者：
Patel, BKC
中科院分区：
文献类型：
--
作者：
Magot, M;Fardeau, ML;Patel, BKC
从中非刚果油田分离到一株专性厌氧螺旋体，命名为SEBR4228(T)(T=模式菌株)。该菌株在37℃(生长温度20-40℃)、pH为7.0-7.2(pH生长范围为5.5-8.0)、氯化钠浓度为5%(氯化钠浓度生长范围为1.0-10%)条件下生长最适。菌株SEBR4228(T)生长在碳水化合物(葡萄糖、果糖、核糖、D-木糖、半乳糖、甘露醇和甘露糖)、甘油、富马酸、多肽和酵母膏上。酵母膏是生长所必需的，不能被维生素取代。它将硫代硫酸盐和硫还原为硫化氢。在硫代硫酸盐存在下，葡萄糖被氧化成乳酸、乙酸盐、二氧化碳和硫化氢，但在没有硫代硫酸盐的情况下，生成乳酸、乙醇、二氧化碳和H-2。将富马酸发酵成乙酸酯和琥珀酸。菌株SEBR4228(T)的G+C含量为50%。菌株SEBR4228(T)呈螺旋形，大小为5-30×0.3-0.5微米，呈螺旋形运动。电子显微镜下可见周质鞭毛呈1-2-1排列。菌株sebr4228(T)具有典型的螺旋毛藻属成员的特征。16S rRNA序列分析表明，该基因与巴氏螺旋藻有较高的同源性(相似性为98.6%)。菌株sebr 4228(T)与巴氏螺旋藻的DNA同源性较低(38%)，并存在其他表型差异，表明该菌株是一个新种，我们将其命名为斯氏螺旋藻。模式菌株为sebr4228(T)(=dsm 11293)。
An obligately anaerobic spirochete designated strain SEBR 4228(T) (T = type strain) was isolated from an oil field of Congo, Central Africa. The strain grew optimally with a sodium chloride concentration of 5% (sodium chloride concentration growth range 1.0-10%) at 37 degrees C (growth temperature range 20-40 degrees C) and pH of 7.0-7.2 (pH growth range pH 5.5-8.0). Strain SEBR 4228(T) grew on carbohydrates (glucose, fructose, ribose, D-xylose, galactose, mannitol and mannose), glycerol, fumarate, peptides and yeast extract. Yeast extract was required for growth and could not be replaced by vitamins. It reduced thiosulfate and sulfur, to H2S. Glucose was oxidised to lactate, acetate, CO2 and H2S in the presence of thiosulfate but in its absence lactate, ethanol, CO2 and H-2 were produced. Fumarate was fermented to acetate and succinate. The G+C content of strain SEBR 4228(T) was 50%. Strain SEBR 4228(T) was spiral shaped measuring 5-30 by 0.3-0.5 mu m and was motile with a corkscrew-like motion. Electron microscopy revealed the presence of periplasmic flagella in a 1-2-1 arrangement. Strain SEBR 4228(T) possessed features typical of the members of the genus Spirochaeta. 16S rRNA sequence analysis revealed that it was closely related to Spirochaeta bajacaliforniensis (similarity 98.6%). The lack of DNA homology with S. bajacaliforniensis (38%), together with other phenotypic differences, indicated that strain SEBR 4228(T) isa new species, which we have designated Spirochaeta smaragdinae. The type strain is SEBR 4228(T) (= DSM 11293).