A strong positive dendritic effect in a peptide dendrimer-catalyzed ester hydrolysis reaction
A strong positive dendritic effect in a peptide dendrimer-catalyzed ester hydrolysis reaction
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DOI：
10.1021/ja044652p
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发表时间：
2004-12-08
影响因子：
15
通讯作者：
Reymond, JL
中科院分区：
文献类型：
--
作者：
Delort, E;Darbre, T;Reymond, JL
树枝状结构在酯水解催化中的贡献是用在所有分支中含有催化共有序列His-Ser的系统性肽树枝状聚合物系列进行研究的。一个强大的积极树突状效应，观察到高达100倍增加组氨酸反应性betweenG 1和G4。动力学研究和等温量热滴定实验表明，强的正树枝化效应是由于结合和催化之间的协同作用。
The contribution of the dendritic structure in catalysis of ester hydrolysis was investigated with a systematic peptide dendrimer series of increasing generation number (G1−G4) containing a catalytic consensus sequence His-Ser in all branches. A strong positive dendritic effect was observed with up to 100-fold increased histidine reactivity betweenG1andG4. Kinetic studies and isothermal calorimetric titration experiments showed that the strong positive dendritic effect resulted from cooperativity between binding and catalysis.