Chloroplast and nuclear genome analysis of the parentage of lemons
Chloroplast and nuclear genome analysis of the parentage of lemons
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DOI：
10.21273/jashs.126.2.210
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发表时间：
2001-03-01
影响因子：
1.9
通讯作者：
Roose, ML
中科院分区：
文献类型：
--
作者：
Gulsen, O;Roose, ML
叶绿体DNA（cpDNA）限制性片段长度多态性被用于研究柠檬[柑橘属柠檬（L.）布尔姆. f.]以及其他几种假定杂种的亲本来源。所研究的30份柑橘属材料包括9个柠檬品种、3个柚子[柚（Burm.） Merrill]、3个香橼（枸橼C. medica L.）、3个柑橘（宽皮柑橘C. reticulata Blanco、克莱门氏小柑橘C. clementina Hort. Ex. Y. Tan.以及酸橘C. sunki Hort. ex. Tan.）以及13个其他分类群的单个代表。使用4种通用叶绿体引物通过聚合酶链式反应扩增出4种不同的柑橘cpDNA片段，扩增产物用4种内切酶（Rsa I、Dra I、Hae III和Mbo I）进行酶切。总共对104种不同的限制性片段进行了评分，并用于简约法系统发育分析。被提议为柑橘属祖先种的3个分类群，即香橼、柚子和柑橘，具有不同的cpDNA模式。柚子为柠檬、甜橙[甜橙（L.）奥斯贝克]和佛手柑（香柠檬C. bergamia Risso and Bolt.）提供了叶绿体基因组，柑橘为粗柠檬（C. jambhiri Lush.）、朗布尔酸橙（C. limonia Osbeck）和‘墨西哥’酸橙[来檬（Christm.）斯温格]提供了叶绿体基因组。数据表明，所研究的这些祖先物种的特定材料并没有直接参与产生这些杂种的杂交事件，因为它们具有相似但不完全相同的cpDNA限制性片段。使用从核基因组DNA扩增的简单重复序列间标记，对包括柚子、香橼、柑橘、酸橙（C. aurantium L.）和‘沙木腰’立花橘（C. micrantha Wester）在内的一组样本进行了作为柠檬可能亲本的检测。酸橙和香橼一起具有在柠檬中发现的核和叶绿体片段，因此被分别提议为许多商业柠檬品种，包括‘里斯本’、‘尤里卡’、‘维拉弗兰卡’和‘莫纳切洛’的母本和父本。
Chloroplast DNA (cpDNA) restriction fragment length polymorphisms were used to study the parentage of lemons [Citrus limon (L.) Burm. f.] and several other putative hybrids. The 30 citrus accessions studied included nine lemons, three pummelos [C. maxima (Burm.) Merrill], three citrons (C. medica L.), three mandarins (C. reticulata Blanco, C. clementina Hort. Ex. Y. Tan., and C. sunki Hort. ex. Tan.), and single representatives of 13 other taxa. Pour different fragments of citrus cpDNA were amplified by polymerase chain reaction using four universal chloroplast primers and amplification products were digested with four endonucleases, Rsa I, Dra I, Hae III, and Mbo I. A total of 104 different restriction fragments were scored and used for phylogenetic analysis by parsimony. The three taxa which have been proposed as ancestral Citrus L. sp., C medica, C. maxima, and C. reticulata, had distinct cpDNA patterns. Pommelo contributed the chloroplast genome to lemons, sweet orange [C. sinensis (L.) Osbeck], and Bergamot orange(C. bergamia Risso and Bolt.), and mandarin contributed the chloroplast genome to rough lemons (C. jambhiri Lush.), Rangpur lime (C. limonia Osbeck), and 'Mexican' lime [C. aurantifolia (Christm.) Swing.]. Data suggest that the particular accessions of these ancestral species that were studied were not directly involved in the hybridization events that created these hybrids, since they have similar but not identical cpDNA restriction fragments. Using inter-simple sequence repeat markers that amplified from nuclear genomic DNA, a set of samples including pummelos, citrons, mandarins, sour oranges (C. aurantium L.), and 'Samuyao' papeda (C,micrantha Wester) were tested as possible parents of lemons. Sour orange and citron together had an nuclear and chloroplast fragments found in lemon and are therefore proposed to be the maternal and paternal parents, respectively, of many commercial lemon cultivars, including 'Lisbon', 'Eureka', 'Villafranca', and 'Monachello'.