Cerebroprotective effects of aminoguanidine in a rodent model of stroke
Cerebroprotective effects of aminoguanidine in a rodent model of stroke
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DOI：
10.1161/01.str.27.8.1393
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发表时间：
1996-08-01
期刊：
影响因子：
8.3
通讯作者：
Tracey, KJ
中科院分区：
文献类型：
--
作者：
Cockroft, KM;Meistrell, M;Tracey, KJ
背景和目的在脑梗塞期间，一系列复杂的细胞毒性事件最终决定了脑细胞损失的数量。本文介绍的研究表明，目前正在进行临床试验的预防糖尿病并发症的实验性治疗药物氨基胍对局灶性脑梗塞具有脑保护作用。方法用氯胺酮麻醉成年Lewis大鼠（每组n = 6至12只），通过串联永久闭塞右侧大脑中动脉和同侧颈总动脉（CCA），然后暂时闭塞对侧CCA来进行局灶性脑梗塞。缺血发作 24 小时后，通过四唑染色的冠状脑切片的平面分析来评估梗塞体积（皮质）。结果 缺血发作 15 分钟后给予氨基胍（320 mg/kg IP）导致梗塞体积显着减少（对照中半球的 7.6+/-2.6%，而氨基胍治疗中半球的 1.3+/-0.2%）大鼠；
Background and Purpose During a cerebral infarction, a complex cascade of cytotoxic events ultimately determines the volume of brain cell loss. The studies presented here demonstrate that aminoguanidine, an experimental therapeutic currently in clinical trials to prevent diabetic complications, is cerebroprotective in focal cerebral infarction.Methods Adult Lewis rats (n=6 to 12 per group)were anesthetized with ketamine and subjected to focal cerebral infarction by tandem permanent occlusion of the right middle cerebral artery and ipsilateral common carotid artery (CCA), followed by temporary occlusion of the contralateral CCA. Infarct volume (cortical) was assessed 24 hours after the onset of ischemia by planimetric analysis of coronal brain slices stained with tetrazolium.Results Aminoguanidine (320 mg/kg IP) administered 15 minutes after the onset of ischemia resulted in a significant reduction of infarct volume (7.6+/-2.6% of hemisphere in controls versus 1.3+/-0.2% of hemisphere in aminoguanidine-treated rats; P