Low-carbohydrate diets adversely impact the skin of a mouse model of photoaging exposed to ultraviolet B radiation.
Low-carbohydrate diets adversely impact the skin of a mouse model of photoaging exposed to ultraviolet B radiation.
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DOI：
10.3164/jcbn.21-18
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发表时间：
2022-01
影响因子：
2.4
通讯作者：
Kaburagi T
中科院分区：
文献类型：
--
作者：
Kanaki K;Otsuka Y;Hino R;Kaburagi T
关于低碳水化合物（LC）饮食影响的研究结果仍然存在争议;因此需要进一步研究以评估其安全性。在这里，我们研究了LC饮食是否会导致C57 BL/6 J小鼠的皮肤损伤。6周龄雌性小鼠（n = 20）自由进食LC（蛋白质/脂肪/碳水化合物能量比= 35：45：20）或对照饮食8周，之后剃去背部毛发，并将一部分小鼠每周暴露于紫外线B辐射三次。 紫外线B照射诱导皮肤表面上的皱纹形成和表皮增厚，这在不存在紫外线B照射的LC饮食喂养的小鼠中也是明显的。同时，经紫外线B照射后，LC饲料喂养的小鼠表皮黑素细胞数量和角质层角化程度增加。肝脏和皮肤的mRNA表达分析显示，紫外线B照射后，LC饮食喂养的小鼠抗氧化酶超氧化物歧化酶1的水平降低。另外，促炎细胞因子，肿瘤坏死因子-α和白细胞介素-1 β的表达，增加响应紫外线B辐射和LC饮食摄入。因此，LC饮食可能会对皮肤形态产生不利影响，并加剧紫外线B照射的影响，这可能与抗氧化剂功能障碍有关。
The study results regarding the effects of low-carbohydrate (LC) diets remain controversial; hence further research is required to assess their safety. Here, we examined whether LC diets cause skin damage in C57BL/6J mice. Six-week-old female mice (n = 20) were fed an LC (protein/fat/carbohydrate energy ratio = 35:45:20) or control diet ad libitum for eight weeks, after which their backs were shaved, and a subset of the mice were exposed to ultraviolet B radiation thrice per week. Ultraviolet B irradiation induced wrinkle formation on the skin surface, and thickening of the epidermis, which was also noticeable in the LC diet-fed mice in the absence of ultraviolet B radiation. Meanwhile, the number of epidermal melanocytes and degree of horny layer keratosis increased in the LC diet-fed mice following ultraviolet B irradiation. mRNA expression analysis of the liver and skin showed decreased levels of the antioxidant enzyme superoxide dismutase 1 following ultraviolet B irradiation only in the LC diet-fed mice. Alternatively, the expression of pro-inflammatory cytokines, tumor necrosis factor-α and interleukin-1β, increased in response to ultraviolet B radiation and LC diet intake. Hence, LC diets may adversely affect skin morphology and exacerbate the effects of ultraviolet B irradiation, which may be associated with antioxidant dysfunction.