Ultrasensitive heterogeneous immunoassay using photothermal deflection spectroscopy
Ultrasensitive heterogeneous immunoassay using photothermal deflection spectroscopy
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DOI：
10.1021/ac00072a016
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发表时间：
1993-12
影响因子：
7.4
通讯作者：
C. Y. Tu;T. Kitamori;T. Sawada;H. Kimura;S. Matsuzawa
中科院分区：
文献类型：
--
作者：
C. Y. Tu;T. Kitamori;T. Sawada;H. Kimura;S. Matsuzawa
提出了一种新的超灵敏、高选择性的免疫分析方法，该方法以金超微粒子为标记材料，利用激光诱导光热偏转进行定量。在实际生物样品的超灵敏激光光谱分析中，背景及其起伏常常会对分析造成干扰。然而，在抗原抗体固相反应中，用聚苯乙烯微球代替传统的硝酸纤维素膜，由于混合蛋白的非特异性吸附速度受到高度抑制，抗原抗体反应面积增加，因此可以充分抑制这种阻碍
A novel ultrasensitive, highly selective immunoassay method that uses gold ultrafine particles as the labeling material and laser-induced photothermal beam deflection quantitation is proposed. Ultrasensitive laser spectrometric analysis of actual biological samples is always obstructed by the background and its fluctuation. However, we could adequately suppress this obstruction by substituting polystyrene microspheres in the antigen-antibody solid-phase reaction instead of the conventional nitrocellulose membrane because the nonspecific adsorption velocity of the admixture proteins was highly restrained and the area of the antigen-antibody reaction was increased