COUPLING OF FLAGELLIN GENE-TRANSCRIPTION TO FLAGELLAR ASSEMBLY IN BACILLUS-SUBTILIS
COUPLING OF FLAGELLIN GENE-TRANSCRIPTION TO FLAGELLAR ASSEMBLY IN BACILLUS-SUBTILIS
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DOI：
10.1128/jb.176.15.4558-4564.1994
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发表时间：
1994-08-01
影响因子：
3.2
通讯作者：
GALIZZI, A
中科院分区：
文献类型：
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作者：
BARILLA, D;CARAMORI, T;GALIZZI, A
通过将lacZ翻译融合到鞭毛蛋白结构基因（hag）中，在体内检查枯草芽孢杆菌中鞭毛蛋白基因表达的调节。我们已经测试了两个已知的突变（flaA 4和flaA 15）的主要鞭毛操纵子和三个删除的影响。一个缺失在fliI顺反子的读框内，一个在fliK顺反子的读框外，最后一个跨越约21 kb的flaA操纵子。在所有情况下，鞭毛蛋白基因的表达是有缺陷的。通过在异丙基-β-D-硫代半乳糖苷（IPTG）诱导型spae启动子的控制下过表达sigD基因，在具有21-kb缺失的菌株中恢复鞭毛蛋白基因表达。这些结果表明，鞭毛蛋白基因的转录是依赖于鞭毛基体的形成，但这种要求可以绕过过表达的sigD。在存在flaD 1、flaD 2和Delta sin突变的情况下也观察到hag表达缺乏。
The regulation of flagellin gene expression in Bacillus subtilis was examined in vivo by means of a lacZ translational fusion to the flagellin structural gene (hag). We have tested the effects of two known mutations (flaA4 and flaA15) in the major flagellar operon and of three deletions. One deletion was in frame in the fliI cistron, one was out of frame in the fliK cistron, and the last spanned about 21 kb of the flaA operon. In all instances, the expression of the flagellin gene was defective. Flagellin gene expression was restored in the strain with the 21-kb deletion by overexpression of the sigD gene under control of the isopropyl-beta-D-thiogalactopy- ranoside (IPTG)-inducible spae promoter. These results indicate that transcription of the flagellin gene is dependent on the formation of the flagellar basal body but that such a requirement can be bypassed by overexpression of sigD. Lack of expression of hag was observed in the presence of flaD1, flaD2, and Delta sin mutations as well.