Ectopic overexpression of wheat adenosine diphosphate-ribosylation factor, TaARF, increases growth rate in Arabidopsis.
Ectopic overexpression of wheat adenosine diphosphate-ribosylation factor, TaARF, increases growth rate in Arabidopsis.
复制标题
小麦腺苷二磷酸核糖基化因子 TaARF 的异位过度表达可提高拟南芥的生长速度。
DOI：
10.1111/j.1744-7909.2008.00792.x
复制
发表时间：
2009
影响因子：
11.4
通讯作者：
Qixin Sun
中科院分区：
文献类型：
--
作者：
Yingyin Yao;Z. Ni;Jinkun Du;Zongfu Han;Yan Chen;Qingbo Zhang;Qixin Sun
杂种与亲本之间的基因表达差异被认为与杂种优势有关。然而，这些差异表达基因的生理功能和可能的杂种优势的贡献是未知的。我们已经分离出一个杂交上调基因编码推定的小麦ADP-核糖基化因子，指定TaARF。实时定量RT-PCR分析结果表明，TaARF基因在根、节和冠中优先表达，杂种中TaARF基因的积累量是双亲的1.5倍以上。为了了解小麦ARF基因可能的作用，TaARF在拟南芥中过量表达，并对转基因植株进行了表征。我们发现，异位过表达的TaARF在拟南芥中导致叶面积增加，增加的生长速度和提前过渡到开花，这表明TaARF在生长和发育中起着重要的作用。本研究提供的证据表明，TaARF在生长发育中起着重要作用，我们推测，该基因的上调表达可能有助于观察到的小麦根和叶生长的杂种优势。
Differential gene expression between hybrids and their parents is considered to be associated with heterosis. However, the physiological functions and possible contribution to heterosis of these differentially expressed genes are unknown. We have isolated one hybrid upregulated gene encoding putative wheat ADP-ribosylation factor, designated TaARF. In this study, real-time quantitative reverse transcription-polymerase chain reaction analysis indicated that the TaARF transcript was preferentially expressed in root, node and crown, and the accumulation of TaARF mRNA in hybrid was more than 1.5-fold higher than that in two parents. In order to understand possible roles of the putative wheat ARF gene, TaARF was overexpressed in Arabidopsis, and the transgenic plants were characterized. We show that ectopic overexpression of TaARF in Arabidopsis leads to increased leaf area, increased growth rate and earlier transition to flowering, suggesting that TaARF plays significant roles in growth and development. This study provides evidence demonstrating that TaARF plays important roles in growth and development and we speculate that the upregulated expression of this gene might contribute to the heterosis observed in wheat root and leaf growth.