Extractive biodecolorization of triphenylmethane dyes in cloud point system by Aeromonas hydrophila DN322p
Extractive biodecolorization of triphenylmethane dyes in cloud point system by Aeromonas hydrophila DN322p
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DOI：
10.1007/s00253-012-4422-4
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发表时间：
2013-07-01
影响因子：
5
通讯作者：
Guo, Jun
中科院分区：
文献类型：
--
作者：
Pan, Tao;Ren, Suizhou;Guo, Jun
三苯基甲烷染料的生物处理是一个重要的课题。大多数微生物的实际应用有限，因为它们不能完全解毒这些染料。本研究通过引入浊点系统，研究了亲水气单胞菌DN322p对三苯甲烷染料的萃取生物脱色。浊点体系由非离子表面活性剂（20g /L） brij30和Tergitol TMN-3按等比例混合而成。结晶紫脱色后，浊点体系的湿细胞重量高于对照体系。薄层色谱结果表明，残留结晶紫及其脱色产物白结晶紫倾向于在凝聚相中分选。因此，实现了对稀相的脱毒，这表明稀相可以在不造成染料污染的情况下排放。同时考察了其他三种三苯基甲烷染料的萃取生物脱色效果。孔雀石绿和亮绿的脱色效果与结晶紫相似。只有乙基紫的脱色率较差，因为DN322p通过吸附对其进行脱色，但没有将其转化为白色。本研究为生物处理三苯基甲烷染料废水提供了潜在的应用前景。
The biological treatment of triphenylmethane dyes is an important issue. Most microbes have limited practical application because they cannot completely detoxicate these dyes. In this study, the extractive biodecolorization of triphenylmethane dyes by Aeromonas hydrophila DN322p was carried out by introducing the cloud point system. The cloud point system is composed of a mixture of nonionic surfactants (20 g/L) Brij 30 and Tergitol TMN-3 in equal proportions. After the decolorization of crystal violet, a higher wet cell weight was obtained in the cloud point system than that of the control system. Based on the results of thin-layer chromatography, the residual crystal violet and its decolorized product, leuco crystal violet, preferred to partition into the coacervate phase. Therefore, the detoxification of the dilute phase was achieved, which indicated that the dilute phase could be discharged without causing dye pollution. The extractive biodecolorization of three other triphenylmethane dyes was also examined in this system. The decolorization of malachite green and brilliant green was similar to that of crystal violet. Only ethyl violet achieved a poor decolorization rate because DN322p decolorized it via adsorption but did not convert it into its leuco form. This study provides potential application of biological treatment in triphenylmethane dye wastewater.