New Insights into the Evolution of Entomopoxvirinae from the Complete Genome Sequences of Four Entomopoxviruses Infecting Adoxophyes honmai, Choristoneura biennis, Choristoneura rosaceana, and Mythimna separata
New Insights into the Evolution of Entomopoxvirinae from the Complete Genome Sequences of Four Entomopoxviruses Infecting Adoxophyes honmai, Choristoneura biennis, Choristoneura rosaceana, and Mythimna separata
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DOI：
10.1128/jvi.00453-13
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发表时间：
2013-05
影响因子：
5.4
通讯作者：
J. Thézé;J. Takatsuka;Zhen Li;J. Gallais;D. Doucet;B. Arif;M. Nakai;E. Herniou
中科院分区：
文献类型：
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作者：
J. Thézé;J. Takatsuka;Zhen Li;J. Gallais;D. Doucet;B. Arif;M. Nakai;E. Herniou
痘病毒是一种核质大DNA病毒，包括两个亚科：脊索痘病毒亚科和昆虫痘病毒亚科，分别感染脊椎动物和昆虫。虽然脊索痘病毒基因组学已被广泛研究，但只有两种昆虫痘病毒（EPV）基因组已被完全测序。我们报道了四种感染鳞翅目昆虫的贝虫痘病毒属的EPV的基因组序列：本麦卷蛾EPV（AHEV）、二叶卷蛾EPV（CBEV）、玫瑰卷蛾EPV（CREV）和粘虫EPV（Mythimna separata EPV）。基因组富含80% AT，长度为228至307 kbp，包含247至334个开放阅读框（ORF）。大多数基因与木斑蛾昆虫痘病毒的基因同源，并编码在末端区域串联重复的几个蛋白质家族。一些基因组还编码与其他昆虫病毒相似的功能未知的蛋白质。比较基因组分析突出了鳞翅目EPV基因组之间的高共线性和与其他痘病毒基因组的基因顺序保守性很小。与先前测序的EPV一样，基因组包括侧接反向末端重复序列的相对保守的中心区域。蛋白质聚类鉴定了104个核心EPV基因。在β昆虫痘病毒中，发现148个核心基因处于相对较高的同线性，表明基因组多样性较低。全基因组和spheroidin基因系统发育分析表明，鳞翅目EPVs在一个单系中的亲缘关系较近，与Betaentomopoxvirus属的亲缘关系较近，且EPVs在昆虫宿主目（鳞翅目、鞘翅目和直翅目）中有明确的划分。这表明EPV与其昆虫宿主的古老共同进化，并且需要修改当前的EPV分类，以将直翅目EPV与鳞翅目特异性β-昆虫痘病毒分开，从而形成一个新的属。
ABSTRACT Poxviruses are nucleocytoplasmic large DNA viruses encompassing two subfamilies, the Chordopoxvirinae and the Entomopoxvirinae, infecting vertebrates and insects, respectively. While chordopoxvirus genomics have been widely studied, only two entomopoxvirus (EPV) genomes have been entirely sequenced. We report the genome sequences of four EPVs of the Betaentomopoxvirus genus infecting the Lepidoptera: Adoxophyes honmai EPV (AHEV), Choristoneura biennis EPV (CBEV), Choristoneura rosaceana EPV (CREV), and Mythimna separata EPV (MySEV). The genomes are 80% AT rich, are 228 to 307 kbp long, and contain 247 to 334 open reading frames (ORFs). Most genes are homologous to those of Amsacta moorei entomopoxvirus and encode several protein families repeated in tandem in terminal regions. Some genomes also encode proteins of unknown functions with similarity to those of other insect viruses. Comparative genomic analyses highlight a high colinearity among the lepidopteran EPV genomes and little gene order conservation with other poxvirus genomes. As with previously sequenced EPVs, the genomes include a relatively conserved central region flanked by inverted terminal repeats. Protein clustering identified 104 core EPV genes. Among betaentomopoxviruses, 148 core genes were found in relatively high synteny, pointing to low genomic diversity. Whole-genome and spheroidin gene phylogenetic analyses showed that the lepidopteran EPVs group closely in a monophyletic lineage, corroborating their affiliation with the Betaentomopoxvirus genus as well as a clear division of the EPVs according to the orders of insect hosts (Lepidoptera, Coleoptera, and Orthoptera). This suggests an ancient coevolution of EPVs with their insect hosts and the need to revise the current EPV taxonomy to separate orthopteran EPVs from the lepidopteran-specific betaentomopoxviruses so as to form a new genus.