Mosquito transcriptome changes and filarial worm resistance in Armigeres subalbatus.
Mosquito transcriptome changes and filarial worm resistance in Armigeres subalbatus.
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DOI：
10.1186/1471-2164-8-463
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发表时间：
2007-12-18
期刊：
影响因子：
4.4
通讯作者：
Christensen, Bruce M
中科院分区：
文献类型：
--
作者：
Aliota, Matthew T;Fuchs, Jeremy F;Mayhew, George F;Chen, Cheng-Chen;Christensen, Bruce M
近白阿蚊是马来丝虫的天然媒介，但它能迅速而熟练地通过黑色素包囊杀死马来丝虫微丝蚴。因为B。malayi和B. pahangi在形态学和生物学上相似，Armigeres-Brugia系统作为研究蚊媒抗性机制的有价值的模型。我们已经在Ar中启动了转录组分析研究。以鉴定参与B的分子组分。马来耐火材料这些初步研究评估了Ar的转录反应。近白肌至B。在感染性血液喂养后1、3、6、12、24、48和72小时的马来人。在这项调查中，我们发起了第一个全面的研究进行抗丝虫免疫反应，以有效地探索免疫反应基因的功能作用后，自然暴露于寄生虫。评估转录反应的研究揭示了未知和保守的未知物、细胞骨架和结构成分以及应激和免疫反应因子的参与。实验结果表明，Ar.因此，黑色素沉着是一个高度复杂的组织特异性过程，涉及与血细胞介导的黑化协同作用的各种效应器反应。这项初步研究为未来的研究提供了基础和方向，这将更充分地剖析这种蚊子-寄生虫系统中抗丝虫免疫应答的性质。该研究还主张继续研究从血细胞和全身产生的RNA，以充分阐明抗丝虫免疫反应的性质。
Armigeres subalbatus is a natural vector of the filarial worm Brugia pahangi, but it rapidly and proficiently kills Brugia malayi microfilariae by melanotic encapsulation. Because B. malayi and B. pahangi are morphologically and biologically similar, the Armigeres-Brugia system serves as a valuable model for studying the resistance mechanisms in mosquito vectors. We have initiated transcriptome profiling studies in Ar. subalbatus to identify molecular components involved in B. malayi refractoriness. These initial studies assessed the transcriptional response of Ar. subalbatus to B. malayi at 1, 3, 6, 12, 24, 48, and 72 hrs after an infective blood feed. In this investigation, we initiated the first holistic study conducted on the anti-filarial worm immune response in order to effectively explore the functional roles of immune-response genes following a natural exposure to the parasite. Studies assessing the transcriptional response revealed the involvement of unknown and conserved unknowns, cytoskeletal and structural components, and stress and immune responsive factors. The data show that the anti-filarial worm immune response by Ar. subalbatus to be a highly complex, tissue-specific process involving varied effector responses working in concert with blood cell-mediated melanization. This initial study provides a foundation and direction for future studies, which will more fully dissect the nature of the anti-filarial worm immune response in this mosquito-parasite system. The study also argues for continued studies with RNA generated from both hemocytes and whole bodies to fully expound the nature of the anti-filarial worm immune response.