Differential regulation of nicotinic acid adenine dinucleotide phosphate and cADP-ribose production by cAMP and cGMP
Differential regulation of nicotinic acid adenine dinucleotide phosphate and cADP-ribose production by cAMP and cGMP
复制标题
DOI：
10.1042/bj3310837
复制
发表时间：
1998-05-01
影响因子：
4.1
通讯作者：
Galione, A
中科院分区：
文献类型：
--
作者：
Wilson, HL;Galione, A
海胆卵被用作研究肌醇1,4,5-三磷酸（IP3）、cadp -核糖（cADPR）以及最近的烟酸-腺嘌呤二核苷酸磷酸（NAADP）诱导的钙释放机制的系统。为了确定cADPR和NAADP是钙释放的内源性信使，必须证明细胞内存在能够代谢这些分子的酶。此外，细胞内cADPR和NAADP的水平应受细胞外刺激的控制。研究表明，cGMP刺激海胆卵中cADPR的合成。本研究表明，海胆卵具有合成和降解NAADP的能力。cADPR和NAADP的合成活性似乎是分开的，具有不同的细胞定位，pH和温度的最佳。我们认为，在海胆卵中，cADPR和NAADP的产生可能受到受体偶联第二信使的不同调节，cADPR的产生受cGMP调节，NAADP的产生受cAMP调节。
The sea urchin egg has been used as a system to study calcium-release mechanisms induced by inositol 1,4,5-trisphosphate (IP3), cADP-ribose (cADPR), and more recently, nicotinic acid-adenine dinucleotide phosphate (NAADP). In order that cADPR and NAADP may be established as endogenous messengers for calcium release, the existence of intracellular enzymes capable of metabolizing these molecules must be demonstrated. In addition, intracellular levels of cADPR and NAADP should be under the control of extracellular stimuli. It has been shown that cGMP stimulates the synthesis of cADPR in the sea urchin egg. The present study shows that the sea urchin egg is capable of synthesizing and degrading NAADP. cADPR and NAADP synthetic activities appear to be separate, with different cellular localizations, pH and temperature optima. We suggest that in the sea urchin egg, cADPR and NAADP production may be differentially regulated by receptor-coupled second messengers, with cADPR production being regulated by cGMP and NAADP production modulated by cAMP.