Neural induction of chick myoblast differentiation in culture.
Neural induction of chick myoblast differentiation in culture.
复制标题
培养中鸡成肌细胞分化的神经诱导。
DOI：
10.1016/0012-1606(82)90223-8
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发表时间：
1982
影响因子：
2.7
通讯作者：
T. Adams
中科院分区：
文献类型：
--
作者：
P. Bonner;T. Adams
发育中的鸡胚腿部肌肉含有一系列克隆独特的成肌细胞。其中一种成肌细胞 CMR-III 在发育的第八天出现，到第 10 天时，其占腿部肌肉组织中所有可克隆细胞的比例高达 50%。发育早期的去神经可以防止 CMR-III 的出现。神经对 CMR-III 产生影响的本质已经通过完全体外系统进行了研究，该系统允许培养的神经元和肌原细胞之间的功能相互作用。来自 4 至 6 天大胚胎的脊髓细胞在明胶包被的培养皿中生长，神经元分化后，将来自 10 至 12 天大去神经胚胎腿部的肌原细胞添加到培养物中。共培养 48 小时后，从平板中取出细胞，并通过克隆分析测定成肌细胞群。共培养物产生 CMR-III 成肌细胞的效率与正常胚胎一样高（高达所有可克隆细胞的 50%），而对照培养物几乎不含 CMR-III。箭毒以及神经元和肌原细胞的物理分离可抑制前体对 CMR-III 的诱导。诱导不是由脊髓细胞释放的可溶或不溶、稳定或不稳定成分介导的。来自骨骼肌、肝脏、肾上腺和心肌的非神经元细胞不会诱导 CMR-III。这表明神经元和敏感的前体肌原细胞之间的接触是前体转化为 CMR-III 所必需的。
Developing chick embryo leg muscle contains a series of clonally distinct myoblasts. One of these myoblast classes, CMR-III, appears during the eighth day of development and, by Day 10, comprises up to 50% of all clonable cells in the leg muscle tissue. Denervation earlier in development prevents the appearance of CMR-III. The nature of neural influence on CMR-III production has been studied with a completelyin vitrosystem which allows functional interaction between cultured neurons and myogenic cells. Spinal cord cells from 4- to 6-day-old embryos are grown on gelatin-coated dishes and, after neuronal differentiation, myogenic cells from the legs of 10- to 12-day-old denervated embryos are added to the cultures. After 48 hr of coculture the cells are removed from the plates and myoblast populations assayed by clonal analysis. Cocultures produce CMR-III myoblasts as efficiently as do normal embryos—up to 50% of all clonable cells—while control cultures remain nearly free of CMR-III. The induction of CMR-III from precursor is inhibited by curare and by physical separation of neurons and myogenic cells. Induction is not mediated by soluble or insoluble, stable or unstable components released by spinal cord cells. Nonneuronal cells from skeletal muscle, liver, adrenal, and heart muscle do not induce CMR-III. It is suggested that contact between neurons and sensitive precursor myogenic cells is required for conversion of precursor into CMR-III.