Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients
Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients
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DOI：
10.1016/s0166-0934(98)00173-6
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发表时间：
1999-03-01
影响因子：
3.1
通讯作者：
Schmidt, CA
中科院分区：
文献类型：
--
作者：
Binder, T;Siegert, W;Schmidt, CA
应用PCR扩增DNA的单链构象多态性分析（SSCP）和人巨细胞病毒（HCMV）糖蛋白B（gB）基因的序列测定，对已知的HCMV毒株进行鉴定，并检测新的病毒变异株。从410例肝移植（LTX）患者队列中研究了61例HCMV PCR阳性患者。SSCP能够区分Davis、AD 169和Towne毒株，此外还能鉴定出5种新的病毒变异株（柏林B、C、E、F和H）。测定了它们的频率、gB和gH型。在长期随访期间观察到同时感染两种或三种毒株或变异株，以及从一种病毒转换为另一种病毒。某些病毒株或gB型的出现与LTX后HCMV感染的明确临床表现之间无相关性。(C)1999 Elsevier Science B.V.保留所有权利。
Single strand conformation polymorphism analysis (SSCP) of PCR-amplified DNA and subsequent DNA sequencing of human cytomegalovirus (HCMV) glycoprotein B (gB) gene were applied to identify known HCMV strains and to detect new virus variants. 61 HCMV PCR positive patients were studied out of a cohort of 410 patients after liver transplantation (LTX). SSCP was able to distinguish between strains Davis, AD169, and Towne, and in addition could identify five new virus variants (Berlin B, C, E, F, and H). Their frequency, gB and gH types were determined. Simultaneous infections with two or three strains or variants, as well as a switch from one virus to another virus were observed during long-term follow-up. No correlation between the occurrence of certain Virus strains or gB types and defined clinical manifestations of HCMV infection after LTX was drawn. (C) 1999 Elsevier Science B.V. All rights reserved.