The crystal structures of DNA Holliday junctions
The crystal structures of DNA Holliday junctions
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DOI：
10.1016/s0959-440x(00)00219-0
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发表时间：
2001-06-01
影响因子：
6.8
通讯作者：
Eichman, BF
中科院分区：
文献类型：
--
作者：
Ho, PS;Eichman, BF
近40年前，Holliday提出了四链复合体或连接作为基因重组一般机制的中心中间体。在过去的两年里，三个不同的研究小组已经确定了这种DNA连接的六个单晶结构。这些结构基本上都采用反平行的Stack-X构象，但可以分为三类：RNA-DNA连接；ACC三核苷酸连接；以及药物诱导的连接。总而言之，这些结构提供了洞察本地和远程相互作用如何有助于在原子水平上定义Holliday结的详细和一般物理特征的洞察。
Nearly 40 years ago, Holliday proposed a four-stranded complex or junction as the central intermediate in the general mechanism of genetic recombination. During the past two years, six single-crystal structures of such DNA junctions have been determined by three different research groups. These structures all essentially adopt the antiparallel stacked-X conformation, but can be classified into three distinct categories: RNA-DNA junctions; ACC trinucleotide junctions; and drug-induced junctions. Together, these structures provide insight into how local and distant interactions help to define the detailed and general physical features of Holliday junctions at the atomic level.