Mouse strain differential neointimal response in vein grafts and wire-injured arteries
Mouse strain differential neointimal response in vein grafts and wire-injured arteries
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DOI：
10.1253/circj.71.1649
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发表时间：
2007-10-01
影响因子：
3.3
通讯作者：
Cooley, Brian C.
中科院分区：
文献类型：
--
作者：
Cooley, Brian C.
背景 临床上在静脉移植和球囊导管插入后都可以看到新内膜发育，但在这两种情况下可能不代表相同的病理。本研究比较了两种小鼠品系：C57B1/6 和 FVB 的静脉移植或动脉损伤移植后的新生内膜增生程度。 方法和结果 将颈静脉分支作为移植物插入同基因匹配小鼠的股动脉中，在 30 天时收获移植物。将线损伤的颈动脉移植到同基因匹配小鼠的颈动脉上，并在第 14 天收获移植物。组织形态计量学显示，静脉移植物在位置依赖性新内膜增厚或管腔横截面积方面没有应变差异。两种菌株在近端移植部位均显示出明显较厚的新内膜和较小的管腔面积（与中部移植物相比；p < 0.05）。相比之下，与 C57B1/6/6 小鼠相比，FVB 小鼠的线损伤颈动脉移植物中的新内膜厚度显着增加 (p < 0.05)。 结论 新内膜形成显示出血管依赖性、应变依赖性差异，FVB 小鼠的动脉新内膜增厚程度更大。这些数据表明，动脉损伤与静脉移植相关的新内膜发育有不同的机制，并且这种差异具有遗传基础。
Background Neointimal development is seen clinically after both vein grafting and balloon catheterization, but may not represent the same pathology under these 2 conditions. This study compared the degree of neointimal hyperplasia after vein grafting or arterial-injury grafts in 2 strains of mice: C57B1/6 and FVB.Methods and Results Jugular vein branches were interpositioned as grafts in the femoral artery of syngenic-matched mice, with graft harvest at 30 days. Wire-injured carotid arteries were grafted to the carotid arteries of syngenic-matched mice, with graft harvest at 14 days. Histomorphometry revealed no strain differences in vein grafts in the extent of position-dependent neointimal thickening or lumen-cross-sectional area. Both strains showed significantly thicker neointima and less lumen area at the proximal graft site (vs the mid-graft; p < 0.05). In contrast, a significantly greater neointimal thickness was found in the wire-injured carotid grafts of FVB mice vs those of C57B1/6/6 mice (p < 0.05).Conclusions Neointimal formation shows a vessel-dependent, strain-dependent difference, with greater arterial neointimal thickening in FVB mice. These data suggest that different mechanisms operate for arterial-injury- vs vein-graft-associated neointimal development and that the difference has a genetic basis.