Screening of genes encoding adhesion factors and biofilm production in methicillin resistant strains of Staphylococcus aureus isolated from Palestinian patients
Screening of genes encoding adhesion factors and biofilm production in methicillin resistant strains of Staphylococcus aureus isolated from Palestinian patients
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DOI：
10.1186/s12864-019-5929-1
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发表时间：
2019-07-12
期刊：
影响因子：
4.4
通讯作者：
Abdeen, Ziad
中科院分区：
文献类型：
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作者：
Azmi, Kifaya;Qrei, Walaa;Abdeen, Ziad
背景金黄色葡萄球菌的细胞间粘附和生物膜的产生使这些细菌对抗菌治疗产生耐药性。在这里，耐甲氧西林金黄色葡萄球菌（MRSA）菌株的特点和流行的基因编码的粘附因子和生物膜形成determined.ResultsAll 248个MRSA菌株头孢西丁光盘扩散确定为阳性的mecA基因。SCCmec-IV是最常见的基因型（92.7%），SCCmec-IVa也很常见（84.3%）。微量滴定板定量分析结果表明，所有菌株均能产生生物膜，其水平从高（21%）到中（46.4%）再到低（32.7%），所有菌株均具有icaD/icaA基因，并能产生生物膜（P
BackgroundIntercellular adhesion and biofilm production by Staphylococcus aureus makes these bacteria resistant to antimicrobial therapy. Here, Methicillin-resistant Staphylococcus aureus (MRSA) strains were characterized and the prevalence of genes encoding adhesion factors and biofilm formation was determined.ResultsAll 248 MRSA isolates identified by cefoxitin disc diffusion were positive for the mecA gene. SCCmec-IV was the most frequently detected genotype (92.7%) and SCCmec-IVa was also very prevalent (84.3%). The quantitative microtiter plate assay showed that all the isolates were able to produce biofilm with levels ranging from high (21%) to moderate (46.4%) to low (32.7%).All the strains possessed the icaD/icaA genes and produced biofilm (P