EVIDENCE FOR A POLARIZED EFFLUX SYSTEM FOR PEPTIDES IN THE APICAL MEMBRANE OF CACO-2 CELLS
EVIDENCE FOR A POLARIZED EFFLUX SYSTEM FOR PEPTIDES IN THE APICAL MEMBRANE OF CACO-2 CELLS
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DOI：
10.1006/bbrc.1993.1114
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发表时间：
1993-02-15
影响因子：
3.1
通讯作者：
HO, NFH
中科院分区：
文献类型：
--
作者：
BURTON, PS;CONRADI, RA;HO, NFH
研究了两种模型肽在人结肠腺癌（Caco-2）细胞融合单层中的转运。在AcPhe(NMePhe)2NH2中，随着根尖室肽浓度的增加，从根尖向基底外侧的转运增加。维拉帕米的存在也增加了转运。相比之下，acphenh2的通量不受维拉帕米的浓度依赖性和影响。此外，在维拉帕米的作用下，AcPhe(NMePhe) 2nh2从基底外侧到根尖方向的转运减慢，而AcPheNH2则保持不变。这些结果与Caco-2细胞中存在一个饱和的、根尖极化的运输系统相一致，该系统阻碍了从根尖向基底外侧的运输，增加了从基底外侧到根尖方向的通量，并显示了这些模型肽的底物特异性。
The transport of two model peptides across confluent monolayers of human colon adenocarcinoma (Caco-2) cells was studied. In the case of AcPhe(NMePhe)2NH2, transport in the apical to basolateral direction was increased with increasing peptide concentration in the apical compartment. Transport was also increased in the presence of verapamil. In contrast, the flux of AcPheNH2was neither concentration dependent nor affected by verapamil. Further, in the presence of verapamil, transport in the basolateral to apical direction was slowed for AcPhe(NMePhe)2NH2and again unchanged for AcPheNH2. These results are consistent with the presence of a saturable, apically polarized transport system in Caco-2 cells which serves to hinder transport in the apical to basolateral direction, increase flux in the basolateral to apical direction and shows substrate specificity for these model peptides.