Cardiac function and blood flow hemodynamics assessment of zebrafish (Danio rerio) using high-speed video microscopy
Cardiac function and blood flow hemodynamics assessment of zebrafish (Danio rerio) using high-speed video microscopy
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DOI：
10.1016/j.micron.2020.102876
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发表时间：
2020-09-01
期刊：
影响因子：
2.4
通讯作者：
Yalcin, Huseyin C.
中科院分区：
文献类型：
--
作者：
Benslimane, Fatiha M.;Zakaria, Zain Z.;Yalcin, Huseyin C.
背景资料：在过去的几十年里，斑马鱼（Danio rerio）被引入作为研究人类疾病的模式生物，包括心血管和神经元疾病。在大多数斑马鱼研究中，需要评估心脏功能和血流动力学以研究干扰对心血管系统的影响。对于心脏功能的评估，最重要的参数包括心率，心输出量，射血分数，面积变化分数，和分数shortening.Methods：一个10秒的高速视频跳动的心脏和流动的血液内的主要血管的斑马鱼受精后不到5天（DPF），通过配备了高速摄像机的体视显微镜记录。使用MicroZebraLab和Image J软件进行分析，用于评估心脏功能。结果：使用这里描述的技术，我们能够简单而有效地评估正常斑马鱼胚胎的心脏功能和血流动力学。我们相信，这里提出的实用方法将有助于心脏研究人员使用斑马鱼作为模型，通过使用实验室中可用的工具来检查心脏功能。
Background: In the last few decades, zebrafish (Danio rerio) were introduced as a model organism to investigate human diseases including cardiovascular and neuronal disorders. In most zebrafish investigations, cardiac function and blood flow hemodynamics need to be assessed to study the effects of the interference on the cardiovascular system. For heart function assessment, most important parameters include heart rate, cardiac output, ejection fraction, fractional area change, and fractional shortening.Methods: A 10 s high-speed video of beating heart and flowing blood within major vessels of zebrafish that are less than 5 days post fertilization (dpf) were recorded via a stereo microscope equipped with a high speed camera. The videos were analyzed using MicroZebraLab and image J software for the assessment of cardiac function.Results: Using the technique described here, we were able to simply yet effectively assess cardiac function and blood flow dynamics of normal zebrafish embryos. We believe that the practical method presented here will help cardiac researchers using the zebrafish as a model to examine cardiac function by using tools that could be available in their laboratory.