Resistance to glyphosate in Lolium rigidum.: I.: Bioevaluation
Resistance to glyphosate in Lolium rigidum.: I.: Bioevaluation
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DOI：
10.1017/s0043174500091992
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发表时间：
1999-07-01
期刊：
影响因子：
2.5
通讯作者：
Krueger, R
中科院分区：
文献类型：
--
作者：
Pratley, J;Urwin, N;Krueger, R
草甘膦是世界上使用最广泛的除草剂。它是非选择性的，在过去的20年里已被用于控制广泛的杂草物种，没有出现抗性杂草生物型。然而，在澳大利亚北方维多利亚的一块田地里，一种生物型的硬直黑麦草（Lolium rigidum）在过去15年里一直使用草甘膦，但未能通过标签推荐的剂量得到控制。根据盆栽剂量反应实验的LD 50值，该生物型表现出对草甘膦的抗性，并且与所测试的敏感生物型相比抗性高出近10倍。该生物型对草甘膦的三种不同盐具有抗性。该生物型对禾草灵的抗性也提高了近三倍，但对其他常用的选择性和广谱除草剂敏感。在两叶和分蘖的发展阶段之间，一个敏感的生物型表现出对草甘膦的耐受性有一个小的但显着的下降，而耐受性的生物型随着年龄的增长保持不变。抗性表型在种子在草甘膦存在下发芽的实验中得到验证。通过对幼苗地上部和根系生长的观察表明，抗性机制可能更多地与地上部有关，而不是与根系有关。
Glyphosate is the world's most widely used herbicide. It is nonselective and has been used to control a broad range of weed species for the past 20 yr, without the appearance of resistant weed biotypes. However, a biotype of Lolium rigidum from a field in Northern Victoria, Australia, in which glyphosate had been used for the past 15 yr, failed to be controlled by label recommended rates. Based on LD50 values from pot dose-response experiments, this biotype exhibited resistance to glyphosate and was nearly 10-fold more resistant compared to the susceptible biotypes tested The biotype was resistant to three different salts of glyphosate. The biotype was also nearly threefold more resistant to diclofop-methyl but was susceptible to other commonly used selective and broad-spectrum herbicides. Between the two-leaf and tillering stages of development, a susceptible biotype exhibited a small but significant decrease in tolerance to glyphosate, whereas tolerance of the resistant biotype remained unchanged with age. The resistant phenotype was verified in experiments in which seed was germinated in the presence of glyphosate. Observations on shoot and root growth of seedlings in these experiments suggested that the resistance mechanism might be associated more with the shoot than with the root.