Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells
Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells
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DOI：
10.1016/j.bbrc.2009.11.113
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发表时间：
2010-01-01
影响因子：
3.1
通讯作者：
Kawai, Toshihisa
中科院分区：
文献类型：
--
作者：
Chen, Weilin;Kajiya, Mikihito;Kawai, Toshihisa
硫化氢(H2S)是一种挥发性硫化物，与炎症有关。然而，尤其是当它是由定植于胃肠道器官的细菌产生的时候，尚不清楚牙周病原体产生的硫化氢是否因此影响了口腔/牙龈上皮细胞介导的炎症反应。本研究的目的是：(1)比较两种细菌体外产生硫化氢的情况。(2)评价H_2S对宿主口腔/牙龈上皮细胞炎症反应的影响牙龈卟啉单胞菌(Pg)在培养过程中产生最多的H_2S，继而促进牙龈和口腔上皮细胞产生促炎细胞因子IL-8。不产生主要可溶性毒力因子的PG突变株。即牙周组织中，仍有硫化氢的产生。以及促进上皮细胞产生IL-8。这些结果表明，PG产生一定浓度的H2S，能够上调牙龈和口腔上皮细胞诱导的IL-8的表达，揭示了可能促进牙周病炎症的可能机制(C)2009 Elsevier Inc.
Hydrogen Sulfide (H2S) a volatile Sulfur compound, is implicated as a cause of inflammation. especially when it is produced by bacteria colonizing gastrointestinal organs However, It IS Unclear if H2S produced by periodontal pathogens affects the inflammatory responses mediated by oral/gingival epithelial cells Therefore. the aims Of this Study were (1) to compare the in vitro production of H2S among. 14 strains of Oral bacteria and (2) to evaluate the effects of H2S on inflammatory response induced in host oral/gingival epithelial cells Porphyromonas gingivalis (Pg) produced the most H2S in Culture, Which, in turn resulted in the promotion of proinflammatory cytokine IL-8 from both gingival and Oral epithelial cells The up-regulation of IL-8 expression was reproduced by the exogenously applied H2S Furthermore. the Mutant Strains of Pg that do not produce major Soluble Virulent factors. ie gingival, still showed the Production Of H2S. as well as the promotion of epithelial IL-8 production. which was abrogated by H2S scavenging reagents These results demonstrated that Pg produces a concentration of H2S capable of Up-regulating-IL-8 expression induced in gingival and oral epithelial cells, revealing a possible mechanism that may promote the inflammation in periodontal disease (C) 2009 Elsevier Inc. All rights reserved