Hyperthermophilic endospores germinate and metabolize organic carbon in sediments heated to 80°C.
Hyperthermophilic endospores germinate and metabolize organic carbon in sediments heated to 80°C.
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DOI：
10.1111/1462-2920.16167
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发表时间：
2022-11
影响因子：
5.1
通讯作者：
Hubert, Casey R. J.
中科院分区：
文献类型：
--
作者：
Bell, Emma;Rattray, Jayne E.;Sloan, Kathryn;Sherry, Angela;Pilloni, Giovanni;Hubert, Casey R. J.
寒冷的表层沉积物是功能多样的嗜热细菌的种子库。这些嗜热菌以内生孢子的形式存在，广泛分布于水生环境中。在这里，我们研究了加热到80°C的冷表层沉积物中内生孢子种群的功能潜力。在80°C下观察到乙酸盐的微生物生产，并且可以通过提供额外的有机碳底物来增强。将来自80°C富集物的16 S rRNA基因扩增子文库与加热至较低温度（50-70°C）的沉积物的16 S rRNA基因扩增子文库进行比较，表明温度选择不同的内生孢子形成细菌群体。尽管硫酸盐还原嗜热菌在50-70°C温育中富集，但80°C超过了它们的耐热性，并选择了在来自加热至90°C的沉积物的扩增子文库中类似地检测到的超嗜热有机营养细菌。基因组解析宏基因组学揭示了共生菌目、热沉降菌科、热单胞菌属和热地生菌属的新型碳循环成员，其具有降解碳水化合物、糖、氨基酸和核苷酸的基因组潜力。嗜热细菌的内生孢子沉积在世界各地的海底沉积物上，它们在埋藏在积累的沉积物中时保持休眠状态。我们的研究结果表明，内生孢子种群可以被激活的温度升高过程中遇到的埋葬，并显示出潜在的有机营养代谢活动，有助于乙酸生成在深热沉积物。
Cold surface sediments host a seedbank of functionally diverse thermophilic bacteria. These thermophiles are present as endospores, which are widely dispersed in aquatic environments. Here, we investigated the functional potential of endospore populations in cold surface sediments heated to 80°C. Microbial production of acetate was observed at 80°C and could be enhanced by supplying additional organic carbon substrates. Comparison of 16S rRNA gene amplicon libraries from 80°C enrichments to sediments heated to lower temperatures (50–70°C) showed that temperature selects for distinct populations of endospore‐forming bacteria. Whereas sulfate‐reducing thermophiles were enriched in 50–70°C incubations, 80°C exceeds their thermal tolerance and selects for hyperthermophilic organotrophic bacteria that are similarly detected in amplicon libraries from sediments heated to 90°C. Genome‐resolved metagenomics revealed novel carbon cycling members of Symbiobacteriales, Thermosediminibacteraceae, Thermanaeromonas and Calditerricola with the genomic potential for the degradation of carbohydrates, sugars, amino acids and nucleotides. Endospores of thermophilic bacteria are deposited on seabed sediments worldwide where they remain dormant as they are buried in the accumulating sediments. Our results suggest that endospore populations could be activated by temperature increases encountered during burial and show the potential for organotrophic metabolic activity contributing to acetate generation in deep hot sediments.
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海底以下 1.5 至 2.4 公里深含煤沉积物的高压富集培养物中存活孢子形成的嗜压细菌的优势
影响因子：
5.2
作者：
Fang J;Kato C;Runko GM;Nogi Y;Hori T;Li J;Morono Y;Inagaki F
通讯作者：
Inagaki F
影响因子：
10.7
作者：
Minh BQ;Nguyen MA;von Haeseler A
通讯作者：
von Haeseler A
影响因子：
3.2
作者：
BONSEN, PPM;SPUDICH, JA;KORNBERG, A
通讯作者：
KORNBERG, A
影响因子：
3.7
作者：
Bushnell B;Rood J;Singer E
通讯作者：
Singer E
影响因子：
16.6
作者：
Beulig F;Schubert F;Adhikari RR;Glombitza C;Heuer VB;Hinrichs KU;Homola KL;Inagaki F;Jørgensen BB;Kallmeyer J;Krause SJE;Morono Y;Sauvage J;Spivack AJ;Treude T
通讯作者：
Treude T