Identification of host and viral factors involved in a dissimilar resolution of a hepatitis C virus infection
Identification of host and viral factors involved in a dissimilar resolution of a hepatitis C virus infection
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DOI：
10.1111/liv.12362
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发表时间：
2014-07-01
影响因子：
6.7
通讯作者：
Quer, Josep
中科院分区：
文献类型：
--
作者：
Cubero, Maria;Gregori, Josep;Quer, Josep
背景和目标：丙型肝炎病毒（HCV）从慢性患者传播到易感个体是研究病毒和宿主因素的好机会，这些因素可能影响丙型肝炎感染的自然过程，无论是自发恢复还是慢性化。比较一个记录在案的丙型肝炎病毒从慢性感染患者到清除感染的受体宿主的性传播瓶颈事件的病例。研究方法：通过直接测序和LightMix分析分别鉴定宿主遗传组分，如I类和II类HLA和IL 28 B多态性（rs 12979860 SNP）。利用大规模焦磷酸测序平台（454 GS-FLX）进行准物种复杂性的深度核苷酸序列分析，并通过ELISPOT表征CD 4特异性免疫应答。结果与结论：测序分析和CD 4应答突出了几个NS 3-解旋酶结构域，其中氨基酸变异性和CD 4免疫应答之间的相互作用可能导致供体患者的慢性化或受体（新感染）患者的病毒清除。
Background & Aims: Hepatitis C virus (HCV) transmission from a chronic patient to a susceptible individual is a good opportunity to study viral and host factors that may influence the natural course of hepatitis C infection towards either spontaneous recovery or chronicity. To compare a documented case of a bottleneck event in the sexual transmission of HCV from a chronically infected patient to a recipient host that cleared infection. Methods: Host genetic components such as Class I and II HLA and IL28B polymorphism (rs12979860 SNPs) were identified by direct sequencing and LightMix analysis, respectively. Deep nucleotide sequence analysis of quasi-species complexity was performed using massive pyrosequencing platform (454 GS-FLX), and the CD4 specific immune response was characterized by ELISPOT. Results and Conclusions: Sequencing analysis and CD4 response highlighted several NS3-helicase domains in which an interplay between amino acid variability and CD4 immune response might have contributed either to chronicity in the donor patient or to viral clearance in the receptor (newly infected) patient.