DNA rearrangement mediated by inverted repeats
DNA rearrangement mediated by inverted repeats
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DOI：
10.1073/pnas.93.2.819
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发表时间：
1996-01-23
影响因子：
11.1
通讯作者：
Liu, LF
中科院分区：
文献类型：
--
作者：
Bi, X;Liu, LF
DNA的反向重复序列广泛存在于真核生物和原核生物的基因组中，并能介导基因组重排。我们发现，反向重复序列可以介导一个有效的和recA独立的重组事件，令人惊讶的是，这种重组的产物不是简单的反向重复序列之间的反向，但几乎完全是一个不寻常的头对头二聚体与复杂的DNA重排。此外，通过增加分隔重复的距离，这种重组显著减少。这些结果可以很容易地解释一个模型，涉及反向重复序列内的DNA复制的前导链和滞后链的相互切换，这导致形成一个霍利迪连接。DNA复制过程中的相互链转换可能是与反向复制相关的基因组重排的一种常见机制。
Inverted repeats of DNA are widespread in the genomes of eukaryotes and prokaryotes and can mediate genome rearrangement, We studied rearrangement mediated by plasmid-borne inverted repeats in Escherichia coli. We show that inverted repeats can mediate an efficient and recA-independent recombination event, Surprisingly, the product of this recombination is not that of simple inversion between the inverted repeats, but almost exclusively an unusual head-to-head dimer with complex DNA rearrangement. Moreover, this recombination is dramatically reduced by increasing the distance separating the repeats. These results can be readily explained by a model involving reciprocal switching of the leading and lagging strands of DNA replication within the inverted repeats, which leads to the formation of a Holliday junction. Reciprocal strand switching during DNA replication might be a common mechanism for genome rearrangement associated with inverted duplication.