Modelling thrombin generation in human ovarian follicular fluid
Modelling thrombin generation in human ovarian follicular fluid
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DOI：
10.1007/s11538-006-9115-7
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发表时间：
2006-11-01
影响因子：
3.5
通讯作者：
Gentry, Rodney D.
中科院分区：
文献类型：
--
作者：
Bungay, Sharene D.;Gentry, Patricia A.;Gentry, Rodney D.
为研究人卵泡液中凝血酶的产生，建立了一个数学模型。模型结果表明，卵泡液中可产生的凝血酶远低于血浆中的凝血酶，未能达到纤维蛋白形成所需的水平，从而支持了卵泡液中凝血酶通过细胞内信号受体启动细胞活动的假设。研究还得出结论，卵泡液中凝血酶生成的扩增途径的缺失是限制凝血酶生成量的主要因素。滴定模型中各种反应物的初始浓度，可以预测维持卵泡中凝血酶产生所需的组织因子和磷脂的量，并得出结论，组织因子途径抑制物对维持凝血酶产生的时间几乎没有影响。通过数值实验来确定卵泡液中的因子V和血栓调节蛋白的作用，这些因子在卵泡液中的水平远低于血浆，这说明了进一步的实验工作的重要性，以确定尚未在文献中报道的几个参数的值。
A mathematical model is constructed to study thrombin production in human ovarian follicular fluid. The model results show that the amount of thrombin that can be produced in ovarian follicular fluid is much lower than that in blood plasma, failing to reach the level required for fibrin formation, and thereby supporting the hypothesis that in follicular fluid thrombin functions to initiate cellular activities via intracellular signalling receptors. It is also concluded that the absence of the amplification pathway to thrombin production in follicular fluid is a major factor in restricting the amount of thrombin that can be produced. Titration of the initial concentrations of the various reactants in the model lead to predictions for the amount of tissue factor and phospholipid that is required to maintain thrombin production in the follicle, as well as to the conclusion that tissue factor pathway inhibitor has little effect on the time that thrombin generation is sustained. Numerical experiments to determine the effect of factor V, which is at a much reduced level in follicular fluid compared to plasma, and thrombomodulin, illustrate the importance for further experimental work to determine values for several parameters that have yet to be reported in the literature.