Protein encoded by the exuperantia gene is concentrated at sites of bicoid mRNA accumulation in Drosophila nurse cells but not in oocytes or embryos.
Protein encoded by the exuperantia gene is concentrated at sites of bicoid mRNA accumulation in Drosophila nurse cells but not in oocytes or embryos.
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由 exuperantia 基因编码的蛋白质集中在果蝇护士细胞中的 bicoid mRNA 积累位点，但不在卵母细胞或胚胎中。
DOI：
10.1101/gad.5.12b.2455
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发表时间：
1991
影响因子：
10.5
通讯作者：
Kilpatrick,M
中科院分区：
文献类型：
--
作者：
Macdonald,PM;Luk,SK;Kilpatrick,M
果蝇中bicoid（bcd）蛋白形态原的前后梯度的形成是bcd mRNA在卵母细胞前极的预定位的结果。bcd mRNA定位需要至少三个基因，其中exuperantia（exu）基因在该途径中最早起作用。我们已经克隆并鉴定了exu基因。值得注意的是，我们发现，exu蛋白与bcd mRNA在本地化的早期阶段，当bcd mRNA位于顶端区域的护士细胞共定位。然而，当bcd mRNA从滋养细胞转移到卵母细胞的前极时，在后期没有共定位。在胚胎中没有检测到exu蛋白。因此，exu仅在启动bcd mRNA定位中起作用，并且在该过程中不起持续作用。
Formation of the anteroposterior gradient of the bicoid (bcd) protein morphogen in Drosophila occurs as a consequence of the prelocalization of bcd mRNA to the anterior pole of the oocyte. bcd mRNA localization requires at least three genes, with the exuperantia (exu) gene acting earliest in the pathway. We have cloned and characterized the exu gene. Notably, we find that exu protein is colocalized with bcd mRNA during the early phase of localization, when bcd mRNA is positioned at the apical regions of the nurse cells. However, there is no colocalization at the later stage when bcd mRNA is transferred from the nurse cells to the anterior pole of the oocyte. There is no detectable exu protein in embryos. Therefore, exu acts only in initiating bcd mRNA localization and does not play a persistent role in that process.