Evaluation of cytotoxic effects and oxidative stress with hydroxyapatite dispersions of different physicochemical properties in rat NR8383 cells and primary macrophages
Evaluation of cytotoxic effects and oxidative stress with hydroxyapatite dispersions of different physicochemical properties in rat NR8383 cells and primary macrophages
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DOI：
10.1016/j.tiv.2009.01.005
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发表时间：
2009-04-01
影响因子：
3.2
通讯作者：
Scheel, Julia
中科院分区：
文献类型：
--
作者：
Albrecht, Catrin;Scherbart, Agnes M.;Scheel, Julia
通过活性氧（ROS）的形成增强细胞毒性和氧化应激进行了讨论，有关纳米材料的潜在危险特性的相关参数。在这项研究中，五种不同尺寸和形态的羟基磷灰石材料的生物相容性，纳米/针状（HA-NN）、纳米/棒状（HA-NR）、纳米/板状（HA-NP）、细/钝针状（HA-FN）和羟基磷灰石-蛋白复合物（HPC）在大鼠NR 8383和原代肺泡巨噬细胞中进行了研究。脂多糖（LPS）和DQ 12石英作为阳性对照。在NR 8383细胞的水溶性四唑盐1（WST-1）和乳酸脱氢酶（LDH）测定中，对于HPC和高达3000 μ g/ml的纯羟基磷灰石样品没有观察到细胞毒性，而HA-FN在LDH测定中在最高剂量下显示出显著效果。在原代细胞中，所有样品在高达300 μ g/ml时均未观察到细胞毒性。电子顺磁共振（EPR）技术测量的活性氧产生显着增加与HA-NN和HPC在NR 8383细胞。在原代细胞中未检测到影响，认为其与生理条件更相关。所有的羟基磷灰石都能引起NR 8383细胞释放TNF-α，但效力明显低于DQ 12石英和LPS。总之，两种细胞类型的综合结果支持纯羟基磷灰石样品以及羟基磷灰石-蛋白质-复合物的良好生物相容性。(c)2009爱思唯尔有限公司保留所有权利。
Enhanced cytotoxicity and oxidative stress through reactive oxygen species (ROS) formation are discussed as relevant parameters regarding potential hazardous properties of nanomaterials. In this study, the biocompatibility of five hydroxyapatite materials of different size and morphology, i.e., nano/needle-shaped (HA-NN), nano/rod-like (HA-NR), nano/plate-like (HA-NP), fine/dull needle-shaped (HA-FN), and a hydroxyapatite-protein-composite (HPC), was investigated in rat NR8383 and primary alveolar macrophages. Lipopolysaccharide (LPS) and DQ12 quartz served as positive controls. In the water-soluble tetrazolium salt 1 (WST-1) and lactate dehydrogenase (LDH) assays with NR8383 cells, no cytotoxicity was observed for HPC and the pure hydroxyapatite samples up to 3000 mu g/ml, while HA-FN showed a significant effect at the highest dose in the LDH assay. In primary cells, no cytotoxicity was observed with all samples up to 300 mu g/ml. ROS generation measured by electron paramagnetic resonance (EPR) technique was significantly enhanced with HA-NN and HPC in NR8383 cells. No effect was detected in primary cells, which are considered more relevant to physiological conditions. All hydroxyapatites elicited TNF-alpha release from the NR8383 cells, but with significantly lower potency than DQ12 quartz and LPS. In conclusion, combined findings in both cell types support a good biocompatibility of the pure hydroxyapatite samples as well as of the hydroxyapatite-protein-composite. (c) 2009 Elsevier Ltd. All rights reserved.