Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle.
Lipid rafts play an important role in the early stage of severe acute respiratory syndrome-coronavirus life cycle.
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DOI：
10.1016/j.micinf.2006.10.015
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发表时间：
2007-01
影响因子：
5.8
通讯作者：
Ikuta K
中科院分区：
文献类型：
--
作者：
Li GM;Li YG;Yamate M;Li SM;Ikuta K
脂筏参与许多病毒的生命周期。在这项研究中，我们发现脂筏在严重急性呼吸综合征（SARS）冠状病毒（CoV）的生命周期中也起着重要作用。用甲基-β-环糊精（MβCD）预处理Vero E6细胞，去除细胞内胆固醇，可抑制SARS CoV颗粒的产生。通过向培养基中添加胆固醇来防止这种抑制，表明病毒颗粒释放的减少是由细胞膜中胆固醇的损失引起的。与此相反，胆固醇消耗后进入阶段（3小时后感染）只造成了有限的影响，病毒颗粒的释放。北方印迹分析显示，MβCD预处理对病毒mRNA水平有显著影响，而感染后3 h处理对病毒mRNA水平无影响。有趣的是，没有明显的证据表明血管紧张素转换酶2与脂筏在Vero E6细胞膜中的共定位。这些结果表明，脂筏可能有助于SARS-CoV在Vero E6细胞的早期复制过程中的感染。
Lipid rafts are involved in the life cycle of many viruses. In this study, we showed that lipid rafts also play an important role in the life cycle of severe acute respiratory syndrome (SARS)-coronavirus (CoV). Cholesterol depletion by pretreatment of Vero E6 cells with methyl-β-cyclodextrin (MβCD) inhibited the production of SARS-CoV particles released from the infected cells. This inhibition was prevented by addition of cholesterol to the culture medium, indicating that the reduction of virus particle release was caused by the loss of cholesterol in the cell membrane. In contrast, cholesterol depletion at the post-entry stage (3 h post-infection) caused only a limited effect on virus particle release. Northern blot analysis revealed that the levels of viral mRNAs were significantly affected by pretreatment with MβCD, but not by treatment at 3 h post-infection. Interestingly, no apparent evidence for colocalization of angiotensin converting enzyme 2 with lipid rafts in the membrane of Vero E6 cells was obtained. These results suggest that lipid rafts could contribute to SARS-CoV infection in the early replication process in Vero E6 cells.
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影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
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影响因子：
5.4
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Thorp, EB;Gallagher, TM
通讯作者：
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影响因子：
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作者：
Yang, ZY;Huang, Y;Nabel, GJ
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影响因子：
5.4
作者：
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通讯作者：
Kielian, M
影响因子：
3.7
作者：
Leser, GP;Lamb, RA
通讯作者：
Lamb, RA