Morphological properties of the occlusion body of Adoxophyes orana granulovirus
Morphological properties of the occlusion body of Adoxophyes orana granulovirus
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Adoxophyes orana颗粒病毒包涵体的形态特征
DOI：
10.1016/j.jip.2018.03.016
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发表时间：
2018
影响因子：
3.4
通讯作者：
Madoka Nakai
中科院分区：
文献类型：
--
作者：
Keiko Tsuruta;Joerg T. Wennmann;Yasuhisa Kunimi;Maki N. Inoue;Madoka Nakai
在 Adoxophyessp 中发现了一种产生具有不寻常形态的包涵体 (OB) 的颗粒病毒 (GV)。日本宫崎县茶田中的（鳞翅目：Tortricidae）幼虫。该分离株被认为是 Adoxophyes 颗粒病毒的突变体，命名为 AdorGV-M，因为其基因组的核苷酸序列与 AdorGV 的英国分离株 AdorGV-E 的核苷酸序列有 99.7% 的同一性。 AdorGV-E 产生典型的卵圆柱形 OB，每个 OB 含有一种包涵体衍生病毒 (ODV)。另一方面，AdorGV-M 产生大型立方体 OB，但每个 OB 的 ODV 数量未知。在本研究中，我们定量了 AdorGV-E 和 -M OB 中的病毒 DNA，并确定了 AdorGV-M OB 中封闭的 ODV 数量。两种分离株的每个 OB 中的病毒 DNA 数量相同，因此我们确认 AdorGV-M 的 1 个 OB 含有 1 个 ODV。探讨脂肪体组织OB形成过程A．通过透射电子显微镜观察接种各分离株的红毛幼虫随时间的变化，并通过显微照片测量 OB 大小。 OB形成的主要区别是AdorGV-M比AdorGV-E需要更多的时间来成熟。在AdorGV-E中，接种后96小时（hpi）ODV开始从一端被卵圆柱形OB覆盖，并且大多数在120hpi时完全闭塞。 AdorGV-M ODV 的闭塞也在 96 hpi 开始，但 OB 形状为立方体。此外，AdorGV-M在120hpi时的OB大小与AdorGV-E相似，但持续增长直至192hpi。 AdorGV-M 因此需要更多时间来完成 OB 形成。因此，AdorGV-E的成熟OB直径为0.22μm，长度为0.39μm，而AdorGV-M的成熟OB直径为1.34×1.23μm。
A granulovirus (GV) that produces occlusion bodies (OBs) having an unusual morphology was found in anAdoxophyessp. (Lepidoptera: Tortricidae) larva in a tea field in Miyazaki Prefecture, Japan. This isolate is considered to be a mutant of Adoxophyes oranagranulovirus, designated AdorGV-M, because the nucleotide sequence of its genome is 99.7% identical to that of an English isolate of AdorGV, AdorGV-E. AdorGV-E produces typical ovocylindrical OBs that contain one occlusion-derived virus (ODV) per OB. On the other hand, AdorGV-M produces large cuboidal OBs, but the number of ODVs per OB was unknown. In this study, we quantified viral DNA in OBs of both AdorGV-E and -M, and determined the number of ODVs occluded in an OB of AdorGV-M. The two isolates had the same quantity of viral DNA in each OB, and we thus confirmed that one OB of AdorGV-M contains one ODV. To investigate the process of OB formation, fat body tissue ofA. honmailarvae inoculated with each isolate was observed in a time course by transmission electron microscopy, and OB sizes were measured from micrographs. The main difference in OB formation was that AdorGV-M required more time to mature than AdorGV-E. In AdorGV-E, ODVs began to be covered from one end with an ovocylindrical OB at 96 h post-inoculation (hpi), and most of them were completely occluded at 120 hpi. Occlusion of AdorGV-M ODVs also began at 96 hpi, but the OB shape was cuboidal. Moreover, the OB size of AdorGV-M was similar to that of AdorGV-E at 120 hpi, but continued to grow until 192 hpi. AdorGV-M thus took more time to complete OB formation. Consequently, AdorGV-E has mature OBs with a diameter 0.22 μm and length 0.39 μm, but those of AdorGV-M are 1.34 × 1.23 μm.