Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate
Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate
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DOI：
10.1007/s00253-012-4058-4
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发表时间：
2012-08-01
影响因子：
5
通讯作者：
O'Connor, Kevin E.
中科院分区：
文献类型：
--
作者：
Kenny, Shane T.;Runic, Jasmina Nikodinovic;O'Connor, Kevin E.
在补料间歇生物反应器中，将PET裂解产物生成的对苯二甲酸钠(TA)和生物柴油生产的废甘油(WG)供应给恶臭假单胞菌GO16。通过改变TA和WG的饲喂顺序，采用了6种饲喂策略。恶臭假单胞菌GO16单独在TA上培养48h，菌体干重可达8.70g/L，PHA达2.61g/L。当TA和WG混合供应时，生物量生产力(g/L/h)比单独供应TA提高1.3~1.7倍，PHA生产力(g/L/h)提高1.8~2.2倍。从TA或WG中积累的PHA的单体组成主要由3-羟基癸酸组成。PHA单体3-羟基十四烯酸和3-羟基十四烯酸不存在于由TA单独积累的PHA中，但在WG添加到发酵中时存在。当WG是发酵的唯一碳源或主要碳源时，积累的PHA相对分子质量低于以TA为唯一底物时积累的PHA。尽管聚合物的性质数据相似，但在PHA积累阶段用WG生产的PHA是粘性的，而在聚合物积累阶段以TA为唯一碳底物生产的PHA在室温下几乎没有粘性。WG与发酵共喂可提高TA的利用率。WG和TA的加料顺序对TA的使用和聚合物性能有影响。
Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.