Temperature Entrainment of the Circadian Cuticle Deposition Rhythm in Drosophila melanogaster
Temperature Entrainment of the Circadian Cuticle Deposition Rhythm in Drosophila melanogaster
复制标题
DOI：
10.1177/0748730410391640
复制
发表时间：
2011-02-01
影响因子：
3.5
通讯作者：
Numata, Hideharu
中科院分区：
文献类型：
--
作者：
Ito, Chihiro;Goto, Shin G.;Numata, Hideharu
在黑腹果蝇（Drosophila melanogaster）中，表皮细胞的外周生物钟控制着表皮细胞的角质层沉积节律，并通过隐色素（crypchrome， CRY）将其带入光-暗（LD）周期。在本研究中，我们研究了温度周期以及温度和温度周期的结合对角质层沉积节律的影响。节律偏向于TC周期，周期为28 h。在21和24 h时，部分个体的节律偏向于TC周期。由于cryb（缺乏功能性的CRY）中的节律被TC周期所携带，因此对于角质层沉积节律的温度夹带并不是必需的。即使在体外培养胸肌或肺泡时，也能实现节律的温度夹带，这表明温度夹带的机制与大脑的中央时钟无关，而热接受的位置位于表皮细胞中。当施加不同周期的LD和TC周期时，LD周期的节律被引入，而TC周期的影响较小。因此，LD周期是一个比TC周期更强的授时合数。LD和TC两种循环处理下，表皮层数的变异减小，且热相与光相重合。因此，我们得出结论，LD和TC周期协同调节节律。即使在体外培养的胸腔中，也观察到LD和TC周期对夹带的协同效应，这表明光和温度信息集成在外周昼夜节律系统中。
The cuticle deposition rhythm, which is observed in the apodeme of the furca in the thorax, is controlled by a peripheral circadian clock in the epidermal cells and entrained to light-dark (LD) cycles via CRYPTOCHROME (CRY) in Drosophila melanogaster. In the present study, we examined the effects of temperature (TC) cycles and the combination of LD and TC cycles on entrainment of the cuticle deposition rhythm. The rhythm was entrained to TC cycles, whose period was 28 h. In T = 21 and 24 h, the rhythm was entrained to TC cycles in some individuals. CRY is not necessary for temperature entrainment of the cuticle deposition rhythm because the rhythm in cryb (lacking functional CRY) was entrained to TC cycles. Temperature entrainment of the rhythm was achieved even when the thoraxes or furcae were cultured in vitro, suggesting that the mechanism for temperature entrainment is independent of the central clock in the brain and the site of the thermoreception resides in the epidermal cells. When LD and TC cycles with different periods were applied, the rhythm was entrained to LD cycles with a slight influence of TC cycles. Thus, the LD cycle is a stronger zeitgeber than the TC cycle. The variance of the number of the cuticle layers decreased in the flies kept under LD and TC cycles with the same period in which the thermophase coincided with the photophase. Therefore, we conclude that LD and TC cycles synergistically entrain the rhythm. Synergistic effects of LD and TC cycles on entrainment were also observed even when the thoraxes were cultured in vitro, suggesting that the light and temperature information is integrated within the peripheral circadian system.