Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle
Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle
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DOI：
10.1007/s00441-006-0308-z
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发表时间：
2007-03-01
影响因子：
3.6
通讯作者：
Sekiya, Ichiro
中科院分区：
文献类型：
--
作者：
Yoshimura, Hideya;Muneta, Takeshi;Sekiya, Ichiro
间充质干细胞（MSC）越来越多地被报道为存在于各种组织中。虽然从人骨髓中获得MSC相对容易，但啮齿动物MSC的分离更加困难，从而限制了体内实验的数量。为了确定合适的细胞来源，我们从骨髓、滑膜、骨膜、脂肪和肌肉中分离大鼠MSCs，并比较它们的产量、扩增和多能性。两次传代后，每个群体中的细胞为CD 11b（-）、CD 45（-）和CD 90（+）。来自滑膜的每个有核细胞的集落殖民地数比来自骨髓的细胞高100倍。就扩增潜力而言，滑膜来源的细胞在集落形成效率、倍数增加和生长动力学方面最高。体外软骨形成试验表明，来自滑膜的颗粒较重，因为它们比来自其他组织的颗粒产生更多的软骨基质，表明它们在软骨形成中的优越性。滑膜来源的细胞在几代后保留了它们的软骨形成潜力。油红-O阳性集落形成率测定显示滑膜和脂肪来源的细胞具有更高的成脂潜力。在钙化过程中，骨膜和肌肉来源的细胞中碱性磷酸酶活性更高。实体组织来源的大鼠间充质干细胞的产量和增殖能力明显优于骨髓来源的。特别是，滑膜衍生的细胞具有最大的增殖和软骨形成的潜力，表明它们在大鼠模型中用于软骨研究的有用性。
Mesenchymal stem cells (MSCs) are increasingly being reported as occurring in a variety of tissues. Although MSCs from human bone marrow are relatively easy to harvest, the isolation of rodent MSCs is more difficult, thereby limiting the number of experiments in vivo. To determine a suitable cell source, we isolated rat MSCs from bone marrow, synovium, periosteum, adipose, and muscle and compared their properties for yield, expansion, and multipotentiality. After two passages, the cells in each population were CD11b (-), CD45 (-), and CD90 (+). The colony number per nucleated cells derived from synovium was 100-fold higher than that for cells derived from bone marrow. With regard to expansion potential, synovium-derived cells were the highest in colony-forming efficiency, fold increase, and growth kinetics. An in vitro chondrogenesis assay demonstrated that the pellets derived from synovium were heavier, because of their greater production of cartilage matrix, than those from other tissues, indicating their superiority in chondrogenesis. Synovium-derived cells retained their chondrogenic potential after a few passages. The Oil Red-O positive colony-rate assay demonstrated higher adipogenic potential in synovium- and adipose-derived cells. Alkaline phosphatase activity was greater in periosteum- and muscle-derived cells during calcification. The yield and proliferation potential of rat MSCs from solid tissues was much better than those from bone marrow. In particular, synovium-derived cells had the greatest potential for both proliferation and chondrogenesis, indicating their usefulness for cartilage study in a rat model.