Preventive effect of S-allylcysteine on lipid peroxides and antioxidants in normal and isoproterenol-induced cardiotoxicity in rats:: A histopathological study
Preventive effect of S-allylcysteine on lipid peroxides and antioxidants in normal and isoproterenol-induced cardiotoxicity in rats:: A histopathological study
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DOI：
10.1016/j.tox.2006.04.039
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发表时间：
2006-07-05
期刊：
影响因子：
4.5
通讯作者：
Prince, P. Stanely Mainzen
中科院分区：
文献类型：
--
作者：
Padmanabhan, M.;Prince, P. Stanely Mainzen
食用富含植物性食品的饮食与降低心血管疾病的风险有关。本研究旨在探讨S-烯丙基半胱氨酸（SAC）在异丙肾上腺素（ISO）诱导的大鼠心肌梗死（MI）中的作用。Wistar大鼠皮下注射ISO（150 mg/kg）后，心肌肌酸激酶、乳酸脱氢酶、天冬氨酸转氨酶和丙氨酸转氨酶等标志酶活性明显降低，血浆和心肌中硫代巴比妥酸反应物和脂质过氧化氢水平明显升高。ISO诱导的大鼠心脏中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽S-转移酶的活性以及血浆和心脏中谷胱甘肽和抗坏血酸的水平也显著降低。口服SAC（100和150毫克/公斤）ISO处理的大鼠每天一次，为期45天，引起显着增加的标志酶的活性，并通过减少脂质过氧化产物和提高抗氧化酶的活性和非酶抗氧化剂的水平，改善了抗氧化状态。SAC对正常大鼠的给药未显示任何显著影响。心肌组织病理学结果显示SAC对ISO处理的大鼠具有保护作用。SAC剂量为150 mg/kg时的效果比剂量为100 mg/kg时更明显，并使所有参数恢复至接近正常。100 mg/kg SAC的作用与α-生育酚（60 mg/kg）相似。本研究结果表明，SAC对ISO诱导的实验性MI具有抗氧化活性。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
The consumption of diets rich in plant foods are associated with a reduced risk of cardiovascular diseases. This study was aimed to evaluate the role of S-allylcysteine (SAC) in isoproterenol (ISO)-induced myocardial infarction (MI) in rats. Subcutaneous injection of ISO (150 mg/kg) to Wistar rats showed a significant decrease in the activities of marker enzymes such as creatine kinase, lactate dehydrogenase, aspartate and alanine transaminases in heart and a significant increase in the levels of thiobarbituric acid reactive substances and lipid hydroperoxides in plasma and heart. ISO-induced rats also showed a significant decrease in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase in heart and the levels of glutathione and ascorbic acid in plasma and heart. Oral administration of SAC (100 and 150 mg/kg) to ISO-treated rats daily for a period of 45 days caused a significant increase in the activities of marker enzymes and improved the antioxidant status by decreasing lipid peroxidative products and increasing the activities of antioxidant enzymes and the levels of nonenzyomic antioxidants. Administration of SAC to normal rats did not show any significant effect. Histopathological findings of the myocardial tissue showed a protective role of SAC in ISO-treated rats. The effect at a dose of 150 mg/kg of SAC was more pronounced than that of the dose 100 mg/kg and brought back all the parameters to near normal. The effect exerted by 100 mg/kg of SAC was similar to that of alpha-tocopherol (60 mg/kg). The results of our study show that SAC possesses antioxidant activity in ISO-induced experimental MI. (c) 2006 Elsevier Ireland Ltd. All rights reserved.