Seed dormancy release in Arabidopsis Cvi by dry after-ripening, low temperature, nitrate and light shows common quantitative patterns of gene expression directed by environmentally specific sensing
Seed dormancy release in Arabidopsis Cvi by dry after-ripening, low temperature, nitrate and light shows common quantitative patterns of gene expression directed by environmentally specific sensing
复制标题
DOI：
10.1111/j.1365-313x.2007.03118.x
复制
发表时间：
2007-07-01
期刊：
影响因子：
7.2
通讯作者：
Hilhorst, Henk W. M.
中科院分区：
文献类型：
--
作者：
Finch-Savage, William E.;Cadman, Cassandra S. C.;Hilhorst, Henk W. M.
种子休眠的深度可能受到许多不同环境信号的影响，但这种明显相似的反应是否有共同的机制还有待研究。全基因组微阵列用于对佛得角岛拟南芥种子进行全局转录分析，这些种子在吸收后暴露于干燥后熟（AR）或低温、硝酸盐和光。发芽研究表明，吸水种子对低温、硝酸盐和光的敏感性取决于收获后 AR 的时间长度。在所有条件下，种子都绝对需要光来完成休眠释放，但这种效果需要暴露于先前的休眠解除环境中。对表达模式的主成分分析观察到分组的生理状态，其方式与种子休眠的深度相关，而不是与环境暴露的类型相关。此外，与休眠或通过 AR 解除休眠相关的基因组转录本丰度的相反变化也与休眠深度有关，并且在不同环境中很常见。除了这些常见的数量变化之外，还描述了休眠解除期间环境特异性基因表达模式。例如，与硝酸盐积累过程相关的基因转录本丰度较高，而硝酸盐减少则与休眠解除相关。在所有条件下的休眠解除过程中，GA3ox1 转录物的数量都会增加，特别是当通过暴露于光来完成休眠解除时。这与脱落酸（ABA）合成相关的转录本形成鲜明对比，脱落酸合成下降。结果与 ABA/赤霉酸平衡在整合解除休眠的环境信号中的作用一致。
The depth of seed dormancy can be influenced by a number of different environmental signals, but whether a common mechanism underlies this apparently similar response has yet to be investigated. Full-genome microarrays were used for a global transcript analysis of Arabidopsis thaliana Cape Verde Island accession seeds exposed to dry after-ripening (AR), or low temperature, nitrate and light when imbibed. Germination studies showed that the sensitivity of imbibed seeds to low temperature, nitrate and light was dependant upon the length of time spent AR following harvest. Seeds had an absolute requirement for light to complete dormancy release in all conditions, but this effect required an exposure to a prior dormancy relieving environment. Principal component analyses of the expression patterns observed grouped physiological states in a way that related to the depth of seed dormancy, rather than the type of environmental exposure. Furthermore, opposite changes in transcript abundance of genes in sets associated with dormancy, or dormancy relief through AR, were also related to the depth of dormancy and common to different environments. Besides these common quantitative changes, environment-specific gene expression patterns during dormancy relief are also described. For example, higher transcript abundance for genes linked to the process of nitrate accumulation, and nitrate reduction was associated with dormancy relief. The quantity of GA3ox1 transcripts increased during dormancy relief in all conditions, in particular when dormancy relief was completed by exposure to light. This contrasts with transcripts linked to abscisic acid (ABA) synthesis, which declined. The results are consistent with a role for the ABA/gibberellic acid balance in integrating dormancy-relieving environmental signals.