Lipid content and fatty acid composition in hepatopancreas and ovaries of Procambrus clarkii during ovarian maturation
Lipid content and fatty acid composition in hepatopancreas and ovaries of Procambrus clarkii during ovarian maturation
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发表时间：
2010
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通讯作者：
G. Leilei
中科院分区：
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作者：
G. Leilei
克氏原螯虾已成为我国水产养殖的主要甲壳类，脂肪是甲壳动物生长的重要营养物质，不仅是能量来源，而且是必需的营养物质。肝胰腺是甲壳动物主要的脂肪储存和加工器官。为了阐明肝胰腺中脂肪的变化规律，研究了肝胰腺中脂肪含量的变化规律，以及肝胰腺中脂肪含量的变化规律。结果表明，肝胰腺中脂肪含量的变化规律与肝胰腺中脂肪含量的变化规律一致，肝胰腺中脂肪含量的变化规律与肝胰腺中脂肪含量的变化规律一致，肝胰腺中脂肪含量的变化规律与肝胰腺中脂肪含量的变化规律一致对克氏原螯虾卵巢成熟过程（分为5个阶段）的脂肪含量和脂肪酸组成、肝胰腺和卵巢的脂肪含量和脂肪酸组成进行了测定，结果表明：（1）卵巢成熟过程中，性腺指数（GSI）从0.12%上升到6.64%，肝胰腺指数（HSI）从7.95%下降到5.31%; (2)尽管子房干物质含量在Ⅰ、Ⅴ期之间从22.29%增加到55.16%，但干物质中脂肪含量仅在Ⅱ期（32.38%）到Ⅲ期（41.90%）有显著增加。(3)肝胰腺中的主要脂肪酸为C16：0、C16：1 n7、C18：0、C18：1 n9、C18：1 n7、C18：2n 6和C18：3 n3，卵巢中的C20：4 n6和C20：5 n3比例也高于肝胰腺，其中C16：0、C18：1 n9和C18：2n 6所占比例较大。(4)卵巢成熟过程中，C20：4 n6、C20：5 n3和C22：6 n3显著减少，而C18：2n 6持续增加。这些结果表明，克氏原螯虾在成熟过程中肝胰腺中的脂质可能转移到卵巢。卵巢成熟后阶段营养物质的积累符合适当的比例。与高度不饱和脂肪酸相比，C18：2n 6可能在卵巢和胚胎发育中发挥更重要的作用。
Procambrus clarkii has become a popular crustacean species for aquaculture in China,and more studies are required on the nutritional requirements of the broodstock during maturation to improve broodstock and egg quality.Lipid is an important nutrient for growth of crustaceans not only as energy sources but also as essential nutrient.Hepatopancreas is regarded as a major lipid storage and processing organ in crustaceans.To clarify the variation of the lipid content and fatty acid composition during ovarian maturation(five stages divided),lipid content and fatty acid composition in hepatopancreas and ovary of Procambrus clarkii were determined at each stage during ovarian maturation.The results indicated that:(1) During ovarian maturation,the gonadosomatic index(GSI) increased dramatically from 0.12% to 6.64%,while the hepatopancreas index(HSI) decreased from 7.95% to 5.31%.(2) Although the dry matter content(%) in ovaries increased from 22.29% to 55.16% between stages Ⅰ and Ⅴ,a significant increase in the levels of lipid content in dry matter was only observed from stage Ⅱ(32.38%) to stage Ⅲ(41.90%).(3) In hepatopancreas,C16:0,C16:1n7,C18:0,C18:1n9,C18:1n7,C18:2n6 and C18:3n3 were the principal fatty acid(FA).Compared to hepatopancreas,ovaries also contained high C20:4n6 and C20:5n3 proportions.Among the principal fatty acid,C16:0,C18:1n9 and C18:2n6 accounted for a large proportion.(4) During ovarian maturation,C20:4n6,C20:5n3 and C22:6n3 decreased significantly,while C18:2n6 increased continuously.These results suggest that lipids in hepatopancreas may be transferred to the ovary of P.clarkii during maturation.The accumulation of nutrients accorded to appropriate proportions at post-stages of ovarian maturation.C18:2n6 may play a more vital role during the ovarian and embryonic development compared to highly unsaturated fatty acid.