Ligands for mixed-mode protein chromatography: Principles, characteristics and design.
Ligands for mixed-mode protein chromatography: Principles, characteristics and design.
复制标题
DOI：
10.1016/j.jbiotec.2009.04.009
复制
发表时间：
2009-10
影响因子：
4.1
通讯作者：
Guofeng Zhao;Xiaoyan Dong;Y. Sun
中科院分区：
文献类型：
--
作者：
Guofeng Zhao;Xiaoyan Dong;Y. Sun
混合模式色谱法是一种利用固定相和进料流中溶质之间多种形式相互作用的色谱方法。与其他类型的色谱相比，混合模式色谱具有不依赖盐的吸附、电荷排斥洗脱和独特的选择性等优点。因此，它已经被证明有利于蛋白质的分离以及其他目的。本文综述了近年来蛋白质纯化的混合模式配体。这些配体通常具有作为疏水部分的脂肪族或芳香族基团和作为离子部分的氨基、羧基或磺酸基。杂环基团因其独特的疏水性和解离性而成为良好的配体候选物。氢键基团对混合模式吸附剂的性能也有影响。在筛选和设计混合模式配体时应考虑这些原则。结合生物信息学和组合学等方法，可以设计合成的亲和配体，用于混合模式的配体设计。因此，需要通过复杂的计算和实验手段来发展合理的混合模式蛋白配体的设计和筛选方法。
Mixed-mode chromatography is a chromatographic method that utilizes more than one form of interactions between the stationary phase and the solutes in a feed stream. Compared with other types of chromatography, mixed-mode chromatography is advantageous in its salt-independent adsorption, facile elution by charge repulsion, and unique selectivity. Hence, it has already proved beneficial for the separation of proteins as well as other purposes. In this article, mixed-mode ligands for protein purification have been reviewed. These ligands usually have an aliphatic or aromatic group as the hydrophobic moiety and an amino, carboxyl or sulfonic group as the ionic moiety. Heterocyclic groups are good ligand candidates for their unique hydrophobicity and dissociation property. Hydrogen bonding groups also have influences on the performance of mixed-mode adsorbents. These principles should be considered in the screening and design of mixed-mode ligands. Strategies for the design of synthetic affinity ligands, especially the bioinformatics and combinatorial methods, may be adopted for mixed-mode ligand design. More efforts are needed for the development of rational design and screening methods for mixed-mode protein ligands by sophisticated computational and experimental approaches.