Inventory, assembly and analysis of Bacillus subtilis ABC transport systems
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems
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DOI：
10.1006/jmbi.1999.2624
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发表时间：
1999-04-02
影响因子：
5.6
通讯作者：
Denizot, F
中科院分区：
文献类型：
--
作者：
Quentin, Y;Fichant, G;Denizot, F
我们已经进行了库存和组装的ATP结合盒（ABC）转运系统的完整基因组的枯草芽孢杆菌。我们将组成ABC转运蛋白的三种蛋白质伴侣（核苷酸结合结构域，NBD;跨膜结构域，MSD;和溶质结合蛋白，SEP）的鉴定与对遗传组织的限制相结合。该策略允许鉴定78种蛋白质中的86种NBD，103种MSD蛋白质和37种SBP。转录单位的分析允许重建59 ABC转运蛋白，其中包括至少一个NBD和一个MSD。一类特殊的五个二聚体ATP酶与MSD伴侣无关，并被认为参与大环内酯类耐药性或调节翻译延伸。此外，我们已经检测到5个基因编码ATP酶没有任何基因编码MSD蛋白在其附近和11个操纵子，只编码膜和溶质结合蛋白。基于这些相似性，提出了三种ATP结合蛋白激活十种不完整系统的设想，表明一种ATP酶可能被一种以上的转运蛋白募集。最后，我们估计了B.枯草芽孢杆菌基因组编码至少78个ABC转运蛋白，已经在38个导入器和40个挤出器中被拆分。根据NBD得到的树以及MSD和SBP的聚类结果，将ABC系统进一步分为11个亚族。与大肠杆菌的比较表明，挤出机在B中的比例过高。枯草杆菌，对应于抗生素和耐药系统的亚家族的扩展。(C)北京：科学出版社.
We have undertaken the inventory and assembly of the ATP binding cassette (ABC) transporter systems in the complete genome of Bacillus subtilis. We combined the identification of the three protein partners that compose an ABC transporter (nucleotide-binding domain, NBD; membrane spanning domain, MSD; and solute-binding protein, SEP) with constraints on the genetic organization This strategy allowed the identification of 86 NBDs in 78 proteins, 103 MSD proteins and 37 SBPs. The analysis of transcriptional units allows the reconstruction of 59 ABC transporters, which include at least one NBD and one MSD. A particular class of five dimeric ATPases was not associated to MSD partners and is assumed to be involved either in macrolide resistance or regulation of translation elongation. In addition, we have detected five genes encoding ATPases without any gene coding for MSD protein in their neighborhood and 11 operons that encode only the membrane and solute-binding proteins. On the bases of similarities, three ATP-binding proteins are proposed to energize ten incomplete systems, suggesting that one ATPase may be recruited by more than one transporter. Finally, we estimate that the B. subtilis genome encodes for at least 78 ABC transporters that have been split in 38 importers and 40 extruders. The ABC systems have been further classified into 11 sub-families according to the tree obtained from the NBDs and the clustering of the MSDs and the SBPs. Comparisons with Escherichia coli show that the extruders are over-represented in B. subtilis, corresponding to an expansion of the sub-families of antibiotic and drug resistance systems. (C) 1999 Academic Press.