Expression of human scavenger receptor B1 on and in human platelets
Expression of human scavenger receptor B1 on and in human platelets
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DOI：
10.1161/01.atv.0000067429.46333.7b
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发表时间：
2003-05-01
影响因子：
8.7
通讯作者：
Ishida, T
中科院分区：
文献类型：
--
作者：
Imachi, H;Murao, K;Ishida, T
目的 - HDL 颗粒的丰度与冠心病的发病率呈负相关。人类清道夫受体 B1 (hSR-B1/CLA-1) 是 HDL 的受体。分别使用逆转录酶-聚合酶链反应和蛋白质印迹测定人血小板中hSR-B1/CLA-1 mRNA和蛋白质的表达。使用流式细胞术显示血小板表面存在该蛋白质。方法和结果-hSR-B1/CLA-1的免疫化学染色表明它在巨核细胞（人骨髓的血小板前体）中表达。这些发现促使我们询问，与对照组相比，从动脉粥样硬化性疾病患者获得的血小板上 hSR-B1/CLA-1 的表达是否存在差异。我们的研究结果表明，动脉粥样硬化性疾病患者血小板表面的 hSR-B1/CLA-1 丰度显着降低。与对照组相比，动脉粥样硬化性疾病患者的 hSR-B1/CLA-1 水平降低与血小板中胆固醇酯含量增加相关。 hSR-B1/CLA-1表达与血小板聚集呈负相关。总之，我们的研究表明 HDL 受体 hSR-B1/CLA-1 在血小板及其前体巨核细胞中表达。 hSR-B1/CLA-1 表达水平与胆固醇酯含量和血小板聚集呈负相关。结论 - 这些发现表明，测定血小板上 hSR-B1/CLA-1 表达水平可能是动脉粥样硬化疾病的有用临床标志物。
Objective - The abundance of HDL particles correlates inversely with the incidence of coronary heart disease. The human scavenger receptor B1 (hSR-B1/CLA-1) is a receptor for HDL. Expression of hSR-B1/CLA-1 mRNA and protein in human platelets was determined using reverse transcriptase - polymerase chain reaction and Western blot, respectively. Presence of the protein on the surface of platelets was shown using flow cytometry.Methods and Results - Immunochemical staining for hSR-B1/CLA-1 showed that it was expressed in megakaryocytes, the platelet precursors of human bone marrow. These findings prompted us to ask whether hSR-B1/CLA-1 was differentially expressed on platelets obtained from patients with atherosclerotic disease compared with those in control subjects. Our findings showed that abundance of hSR-B1/CLA-1 was significantly reduced on the surface of platelets from patients with atherosclerotic disease. The reduced levels of hSR-B1/CLA-1 were associated with increased cholesterol ester content in platelets from patients with atherosclerotic disease compared with control subjects. A negative correlation existed between hSR-B1/CLA-1 expression and platelet aggregation. In summary, our studies show that the HDL receptor hSR-B1/CLA-1 is expressed in platelets and their precursor, the megakaryocyte. The levels of hSR-B1/CLA-1 expression correlate inversely with cholesterol ester content and platelet aggregation.Conclusion - These findings suggest that determining the level of hSR-B1/CLA-1 expression on the platelets may be a useful clinical marker for atherosclerotic diseases.