Circadian expression of clock genes in human peripheral leukocytes
Circadian expression of clock genes in human peripheral leukocytes
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DOI：
10.1016/j.bbrc.2007.01.063
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发表时间：
2007-03-23
影响因子：
3.1
通讯作者：
Yokoyama, Mitsuhiro
中科院分区：
文献类型：
--
作者：
Fukuya, Hiroyuki;Emoto, Noriaki;Yokoyama, Mitsuhiro
在哺乳动物中，行为和生理过程显示由昼夜节律系统调节的24小时节律。在本研究中，我们调查的可能性，时钟基因在外周血白细胞的表达可以用来评估昼夜节律钟系统。我们发现Per 1和Per 2在小鼠外周血白细胞的mRNA表达中表现出昼夜振荡。此外，Per 1和Per 2 mRNA在外周血白细胞中表达的节律在已知具有废除的生物钟的纯合CryI/2双缺陷小鼠中严重钝化。我们研究了人类白细胞中时钟基因的昼夜节律表达，发现Per 1 mRNA表现出强的昼夜节律表达，而Per 2和Bmall mRNA表现出弱的节律。这些观察结果表明，监测Per 1 mRNA在人类白细胞中的表达可能是有用的调查生理和病理生理状态的昼夜节律系统的功能。(c)2007爱思唯尔公司All rights reserved.
In mammals, behavioral and physiological processes display 24-h rhythms that are regulated by a circadian system. In the present study, we investigated the possibility that the expression of clock genes in peripheral leukocytes can be used to assess the circadian clock system. We found that Per1 and Per2 exhibit circadian oscillations in mRNA expression in mouse peripheral leukocytes. Furthermore, the rhythms of Per1 and Per2 mRNA expression in peripheral leukocytes are severely blunted in homozygous CryI/2 double-deficient mice that are known to have an abolished biological clock. We have examined the circadian expression of clock genes in human leukocytes and found that Per1 mRNA exhibits a robust circadian expression while Per2 and Bmall mRNA showed weak rhythm. These observations suggest that monitoring Per1 mRNA expression in human leukocytes may be useful for investigating the function of the circadian system in physiological and pathophysiological states. (c) 2007 Elsevier Inc. All rights reserved.