Ca2+ signal contributing to the synthesis and emission of monoterpenes regulated by light intensity in Lilium 'siberia'
Ca2+ signal contributing to the synthesis and emission of monoterpenes regulated by light intensity in Lilium 'siberia'
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DOI：
10.1016/j.plaphy.2015.03.005
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发表时间：
2015-06-01
影响因子：
6.5
通讯作者：
Leng, Pingsheng
中科院分区：
文献类型：
--
作者：
Hu, Zenghui;Li, Tianjiao;Leng, Pingsheng
花香是植物挥发性物质的重要组成部分，受环境因素的影响。西伯利亚百合单萜类化合物的释放受光照强度的调控，但其调控机制尚不清楚。本研究以西伯利亚百合（Lilium 'siberia'）花被片为材料，研究了不同光照强度（0、100、300、600、1000和1500 μ mol·m（-2）·s（-1））下，花被片中单萜合成酶基因Li-mTPS的表达和净Ca ~（2+）通量。此外，在600 μ mol m（-2）s（-1）下，检测了LaCl 3和乙二醇-双-（2-氨基乙基醚）-N，N，N '，N'-四乙酸（EGTA）对Li-mTPS表达、单萜排放和净Ca 2+通量的影响。结果表明，沿着光照强度的增强，Li-mTPS的表达量逐渐增加，细胞内净Ca 2+内流也随之增加，呈现出相似的规律。结果表明，LaCl 3和EGTA能有效抑制光诱导的Li-mTPS表达的增加和细胞内钙内流的增加。LaCl 3和EGTA处理后，单萜类化合物的释放量显著降低。可见，Ca 2+信号参与了光强度对西伯利亚杏花被片单萜类化合物合成和释放的调控。光照强度的增加首先引发Ca 2+内流到细胞质中，然后激活下游单萜类脱氢酶基因的表达，从而调控单萜类化合物的合成和释放。但在信号转导途径中，还存在其他的光调控机制，有待于进一步研究。(C)2015年Elsevier Masson SAS。All rights reserved.
The floral scent is an important part of plant volatile compounds, and is influenced by environmental factors. The emission of monoterpenes of Lilium 'siberia' is regulated by light intensity, but the mechanism is large unknown. In this study, the expression of Li-mTPS, a monoterpene synthase gene in the tepals of Lilium 'siberia', and net Ca2+ flux were investigated after exposure to different levels of light intensity (0, 100, 300, 600, 1000, and 1500 mu mol m(-2) s(-1)). Moreover the effect of LaCl3 and ethylene glycol-bis-(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) on the Li-mTPS expression, monoterpene emission, and net Ca2+ flux were examined at 600 mu mol m(-2) s(-1). The results showed that along with the enhancement of light intensity, the expression level of Li-mTPS increased gradually, and the net Ca2+ influx was also enhanced showing a similar pattern. It was found that LaCl3 and EGTA effectively inhibited the increase in expression of Li-mTPS and the net Ca2+ influx induced by light treatment. Moreover, the release amounts of monoterpenes decreased significantly after treatment with LaCl3 and EGTA. So it can be concluded that Ca2+ signal contributed to the biosynthesis and emission of monoterpenes regulated by light intensity in Mum 'siberia' tepals. The increased light intensity firstly triggered the Ca2+ influx to cytoplasm, and then the gene expression of monoterpene synthases downstream was activated to regulate the biosynthesis and emission of monoterpenes. But in the signaling pathway other mechanisms were thought to be involved in the emission of monoterpenes regulated by light intensity, which need to be investigated in future research. (C) 2015 Elsevier Masson SAS. All rights reserved.