Breast Cancer Associated Fibroblasts Promote MCF-7 Invasion in vitro by Secretion of HGF
Breast Cancer Associated Fibroblasts Promote MCF-7 Invasion in vitro by Secretion of HGF
复制标题
乳腺癌相关成纤维细胞通过分泌 HGF 促进 MCF-7 体外侵袭
DOI：
10.1007/s11596-012-0016-8
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发表时间：
2012-02-01
影响因子：
--
通讯作者：
Chen, Gang
中科院分区：
文献类型：
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作者：
Chen, Pingbo;Mo, Qingqing;Chen, Gang
本研究旨在探讨乳腺癌相关成纤维细胞（CAF）对乳腺癌细胞系MCF-7迁移和侵袭的影响，并探讨肝细胞生长因子（HGF）是否参与该过程。通过胶原酶消化获得原代乳腺 CAF 及其相应的正常乳腺成纤维细胞 (NF)。在此共培养的基础上，利用Transwell比较CAFs和NFs之间MCF-7细胞的迁移和侵袭能力。通过ELISA检测两者之间HGF表达的差异。通过在 CAF 中使用 RNA 干扰技术来抑制 HGF 的分泌。然后通过Transwell检测MCF-7细胞迁移和侵袭能力的变化。最终，我们分离出了高纯度的CAF和NF，并且CAF比NF具有更强的促进MCF-7迁移和侵袭的能力。 ELISA结果表明，CAFs分泌较高的HGF，通过RNA干扰敲除CAFs中HGF的分泌后，MCF-7迁移和侵袭的能力下降。提示CAFs在体外可以通过HGF促进MCF-7的迁移和侵袭。
This study was aimed to explore the influence of breast cancer associated fibroblasts (CAFs) in migration and invasion of breast cancer cell line MCF-7, and investigate whether hepatocyte growth factor (HGF) is involved in this process. Primary breast CAFs and their corresponding normal breast fibroblasts (NFs) were obtained by collagenase digestion. On the basis of the co-culture, the migration and invasion capacity of MCF-7 cells was compared between CAFs and NFs by Transwell. The difference in the HGF expression between them was detected by ELISA. The secretion of HGF was knocked down by using RNA interference technology in CAFs. Then the changes of migration and invasion capacity of MCF-7 cells were investigated by Transwell. Eventually, we isolated high-purity CAFs and NFs, and the CAFs had a stronger ability in promoting MCF-7 migration and invasion than the NFs. ELISA results demonstrated that CAFs secreted higher HGF, and the capacity of MCF-7 migration and invasion was declined after knocking down the secretion of HGF in CAFs by RNA interference. It is suggested that CAFs can promote MCF-7 migration and invasion through HGF in vitro.