Bioaerosol sampling for the detection of aerosolized influenza virus
Bioaerosol sampling for the detection of aerosolized influenza virus
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DOI：
10.1111/j.1750-2659.2007.00020.x
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发表时间：
2007-05-01
影响因子：
4.4
通讯作者：
Beezhold, Don H.
中科院分区：
文献类型：
--
作者：
Blachere, Francoise M.;Lindsley, William G.;Beezhold, Don H.
流感病毒被用来表征基于旋风的两级个人生物气溶胶采样器的功效，用于收集和分离雾化病毒颗粒的大小。方法采用Collison单喷雾化器将流感病毒(R)减毒疫苗雾化到静气沉降室中。病毒颗粒是用生物气溶胶采样器捕获的，该采样器利用2个微离心管收集空气中的颗粒。第一管（T1）收集直径大于1.8 μ m的颗粒，第二管（T2）收集直径在1.0 ~ 1.8 μ m之间的颗粒，备用过滤器(F)收集亚微米级的颗粒。雾化后，采用定量PCR检测并定量H1N1和H3N2流感病毒株。结果基于qPCR结果，我们证明了使用两级生物气溶胶采样器可以有效地收集和分离雾化的病毒颗粒。大部分病毒颗粒在生物气溶胶采样器的T2 （1 ~ 1.8 μ m）和备用过滤器（< 1 μ m）上收集。此外，我们发现两级采样器对病毒颗粒的检测与收集时间成正比。因此，与5分钟、10分钟和20分钟气溶胶收集点相比，40分钟的病毒颗粒计数明显更高。结论由于缺乏经验数据，流感的气溶胶传播经常受到质疑。使用fluumist (R)，我们证明了一种新开发的生物气溶胶采样器能够回收和分离雾化的病毒颗粒。该采样器应成为临床环境中研究病毒传播的重要工具，并可能对了解流感病毒传播模式作出重大贡献。
Background Influenza virus was used to characterize the efficacy of a cyclone-based, two-stage personal bioaerosol sampler for the collection and size fractionation of aerosolized viral particles.Methods A Collison single-jet nebulizer was used to aerosolize the attenuated FluMist (R) vaccine into a calm-air settling chamber. Viral particles were captured with bioaerosol samplers that utilize 2 microcentrifuge tubes to collect airborne particulates. The first tube (T1) collects particles greater than 1.8 mu m in diameter, while the second tube (T2) collects particles between 1.0 and 1.8 mu m, and the back-up filter (F) collects submicron particles. Following aerosolization, quantitative PCR was used to detect and quantify H1N1 and H3N2 influenza strains.Results Based on qPCR results, we demonstrate that aerosolized viral particles were efficiently collected and separated according to aerodynamic size using the two-stage bioaerosol sampler. Most viral particles were collected in T2 (1-1.8 mu m) and on the back-up filter (< 1 mu m) of the bioaerosol sampler. Furthermore, we found that the detection of viral particles with the two-stage sampler was directly proportional to the collection time. Consequently, viral particle counts were significantly greater at 40 minutes in comparison to 5, 10 and 20 minute aerosol collection points.Conclusions Due to a lack of empirical data, aerosol transmission of influenza is often questioned. Using FluMist (R), we demonstrated that a newly developed bioaerosol sampler is able to recover and size fractionate aerosolized viral particles. This sampler should be an important tool for studying viral transmission in clinical settings and may significantly contribute towards understanding the modes of influenza virus transmission.