Genetic mapping of seed shape in three populations of recombinant inbred lines of soybean (Glycine max L. Merr.)
Genetic mapping of seed shape in three populations of recombinant inbred lines of soybean (Glycine max L. Merr.)
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DOI：
10.1007/s00122-006-0392-1
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发表时间：
2006-11-01
影响因子：
5.4
通讯作者：
Mansur, L.
中科院分区：
文献类型：
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作者：
Salas, P.;Oyarzo-Llaipen, J. C.;Mansur, L.
圆形大豆种子是食品型大豆的抢手货。此外，种子几何形状的遗传控制也具有科学意义。本研究的目的是估计种子形状性状的遗传力和定位数量性状基因座（QTL）。三个密集映射的重组近交群体，每个192个分离子，Minsoy x阿彻，Minsoy x Noir 1，和Noir 1 x阿彻。在Los安第斯山脉（智利）和东兰辛（密歇根州）（美国）进行了一项双代表双地点实验。测量种子高度（SH）、宽度（SW）、长度（SL）和作为宽度X高度X长度的种子体积（SV）以确定种子形状。用方差分量分析法估计遗传力。在10个连锁群（LG）中共检测到19个显著性QTL（LOD>= N3.7）。只有一个QTL是稳定的群体和环境中，6个稳定的至少两个群体在两个环境中。单个QTL解释的表型变异量从SH的7.5%到SW的18.5%不等，至少有30%的性状遗传变异由4个或更少的QTL控制，所有性状在所有群体中均高度相关，相关值在0.5 - 0.9之间，除SL和SW在所有群体中不显著相关或相关性低外。所有性状的狭义遗传力范围为0.42 - 0.88。LG u9、u11和u14是各性状QTL的基因组热点。QTL的数量和基因组分布证实了种子形状的复杂遗传控制。所有性状的超亲分离表明，仔细选择具有相似的表型，但不同的基因型，使用分子标记的父母可以导致理想的超亲分离。
Round soybean seeds are sought-after for food-type soybean. Also the genetic control of seed geometry is of scientific interest. The objectives of this study were to estimate heritability and map quantitative trait loci (QTLs) responsible for seed shape traits. Three densely mapped recombinant inbred populations each with 192 segregants were used, Minsoy x Archer, Minsoy x Noir1, and Noir1 x Archer. A two rep two location experiment was conducted in Los Andes, Chile, and East Lansing, MI, USA. Seed height (SH), width (SW), length (SL), and seed volume (SV) as width x height x length were measured to determine seed shape. Heritability was estimated by variance component analysis. A total of 19 significant QTLs (LOD >= N 3.7) in ten linkage groups (LG) were detected for all the traits. Only one QTL was stable across populations and environments and six were stable in at least two populations in both environments. The amount of phenotypic variation explained by a single QTL varied from 7.5% for SH, to 18.5% for SW and at least 30% of the genetic variation for the traits is controlled by four QTL or less. All traits were highly correlated with each other in all populations with values ranging from 0.5 to 0.9, except for SL and SW that were not significantly correlated or had a low correlation in all populations. Narrow sense heritabilities for all traits ranged from 0.42 to 0.88. We note that LG u9, u11, and u14 are hot points of the genome for QTLs for various traits. The number and genomic distribution of the QTLs confirms the complex genetic control of seed shape. Transgressive segregation was observed for all traits suggesting that careful selection of parents with similar phenotypes but different genotypes using molecular markers can result in desirable transgressive segregants.