Evidence for multiple interspecific hybridization in Saccharomyces sensu stricto species.
Evidence for multiple interspecific hybridization in Saccharomyces sensu stricto species.
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DOI：
10.1016/s1567-1356(01)00051-4
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发表时间：
2002-01-01
影响因子：
3.2
通讯作者：
Ganter, Philip F
中科院分区：
文献类型：
--
作者：
de Barros Lopes, Miguel;Bellon, Jennifer R;Ganter, Philip F
荧光扩增片段长度多态性分析表明严格意义上的酵母属物种之间的基因交换水平很高，一些菌株经历了多次种间杂交事件，随后基因组复杂性发生了变化。两个啤酒菌株被证明是酿酒酵母和异源倍体物种巴斯德酵母之间的杂交种。贝酵母的模式菌株CBS 380（T）的基因组结构也与S. pastorianus基因转移结果表明，苹果酒酵母，CID1，拥有核DNA从三个不同的物种。交配实验表明，单倍体细胞的种间接合没有障碍。此外，异源多倍体菌株能够与其他严格意义上的酵母进行进一步杂交。这些结果表明，严格意义上的酵母菌种之间的基因渗入是可能的。
Fluorescent amplified fragment length polymorphism analysis demonstrates a high level of gene exchange between Saccharomyces sensu stricto species, with some strains having undergone multiple interspecific hybridization events with subsequent changes in genome complexity. Two lager strains were shown to be hybrids between Saccharomyces cerevisiae and the alloploid species Saccharomyces pastorianus. The genome structure of CBS 380(T), the type strain of Saccharomyces bayanus, is also consistent with S. pastorianus gene transfer. The results indicate that the cider yeast, CID1, possesses nuclear DNA from three separate species. Mating experiments show that there are no barriers to interspecific conjugation of haploid cells. Furthermore, the allopolyploid strains were able to undergo further hybridizations with other Saccharomyces sensu stricto yeasts. These results demonstrate that introgression between the Saccharomyces sensu stricto species is likely.