Flavanol-rich cocoa induces nitric-oxide-dependent vasodilation in healthy humans
Flavanol-rich cocoa induces nitric-oxide-dependent vasodilation in healthy humans
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DOI：
10.1097/00004872-200312000-00016
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发表时间：
2003-12-01
影响因子：
4.9
通讯作者：
Hollenberg, NK
中科院分区：
文献类型：
--
作者：
Fisher, NDL;Hughes, M;Hollenberg, NK
背景 饮用富含类黄酮的饮料，包括茶和红酒，与冠状动脉事件的减少有关，但其生理机制仍不清楚。可可含有极高浓度的黄烷醇，黄烷醇是一种类黄酮亚类，可在体外激活一氧化氮合酶。 目的 检验富含黄烷醇的可可可诱导人体一氧化氮依赖性血管舒张的假设。 设计 该研究使用时间和饮料对照，前瞻性评估了富含黄烷醇的可可的效果。参与者对干预不知情；方法 使用体积敏感、经过验证的校准体积描记器测量 27 名健康人在食用富含黄烷醇的可可 [821 毫克黄烷醇/天，定量为 (-)-表儿茶素和相关原花青素低聚物] 5 天之前和之后的手指脉搏波振幅。特定一氧化氮合酶抑制剂 N-G-硝基-L-精氨酸甲酯 (L-NAME) 在第 1 天（在可可之前）和第 5 天（在急性摄入可可之后）静脉注射。结果 富含黄烷醇的可可的四天诱导了一致且显着的外周血管舒张（P = 0.009）。第 5 天，脉搏波振幅对可可表现出更大的额外急性反应（P = 0.01）。 L-NAME 完全逆转了这种血管舒张作用（P = 0.004）。此外，摄入富含黄烷醇的可可可增强血管舒张剂对缺血的反应。缺乏黄烷醇的可可引起的反应要小得多（P = 0.005），并且在时间对照研究中没有引起任何反应。富含黄烷醇的可可还增强了 L-NAME 的全身升压作用（P = 0.005）。 结论 在健康人类中，富含黄烷醇的可可通过激活一氧化氮系统诱导血管舒张，为富含黄烷醇的食物诱导的冠状动脉事件提供了一种合理的保护机制。 (c) 2003 年利平科特·威廉姆斯和威尔金斯。
Background Consumption of flavonoid-rich beverages, including tea and red wine, has been associated with a reduction in coronary events, but the physiological mechanism remains obscure. Cocoa can contain extraordinary concentrations of flavanols, a flavonoid subclass shown to activate nitric oxide synthase in vitro.Objective To test the hypothesis that flavanol-rich cocoa induces nitric-oxide-dependent vasodilation in humans.Design The study prospectively assessed the effects of Flavanol-rich cocoa, using both time and beverage controls. Participants were blinded to intervention; the endpoint was objective and blinded.Methods Pulse wave amplitude was measured on the finger in 27 healthy people with a volume-sensitive validated calibrated plethysmograph, before and after 5 days of consumption of Flavanol-rich cocoa [821 mg of flavanols/day, quantitated as (-)-epicatechin, and related procyanidin oligomers]. The specific nitric oxide synthase inhibitor, N-G-nitro-L-arginine methyl ester (L-NAME) was infused intravenously on day 1, before cocoa, and on day 5, after an acute ingestion of cocoa.Results Four days of flavanol-rich cocoa induced consistent and striking peripheral vasodilation (P = 0.009). On day 5, pulse wave amplitude exhibited a large additional acute response to cocoa (P = 0.01). L-NAME completely reversed this vasodilation (P = 0.004). In addition, intake of flavanol-rich cocoa augmented the vasoclilator response to ischemia. Flavanol-poor cocoa induced much smaller responses (P = 0.005), and none was induced in the time-control study. Flavanol-rich cocoa also amplified the systemic pressor effects Of L-NAME (P = 0.005).Conclusion In healthy humans, flavanol-rich cocoa induced vasodilation via activation of the nitric oxide system, providing a plausible mechanism for the protection that flavanol-rich foods induce against coronary events. (c) 2003 Lippincott Williams & Wilkins.