DEVELOPMENTAL EXPRESSION OF THE ARABIDOPSIS CYCLIN GENE CYC1AT
DEVELOPMENTAL EXPRESSION OF THE ARABIDOPSIS CYCLIN GENE CYC1AT
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DOI：
10.1105/tpc.6.12.1763
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发表时间：
1994-12-01
期刊：
影响因子：
11.6
通讯作者：
INZE, D
中科院分区：
文献类型：
--
作者：
FERREIRA, PCG;HEMERLY, AS;INZE, D
在真核生物中，细胞周期进程的控制是由细胞周期依赖性激酶相关亚基 (Cdc2) 和细胞周期蛋白亚基组成的异聚蛋白激酶复合物进行的。为了探讨细胞周期蛋白转录在细胞分裂的发育调控中发挥作用的可能性，我们检测了拟南芥中细胞周期蛋白基因 (cyc1At) 的空间和时间表达。在根和芽顶端分生组织以及胚胎发生过程中，cyc1At 表达几乎完全局限于分裂细胞。在根分生组织中观察到 cyc1At 表达的细胞特异性模式。我们检查了诱导分化细胞的细胞分裂对 cyc1At 表达的影响。在侧根形成过程中，cyc1At 表达的诱导是一个非常早期的事件，并且在解剖学改变可见之前就被检测到。用安磺灵处理根可阻止中期细胞分裂，但不会抑制生长素对 cyc1At 的诱导，这表明 cyc1At 表达的诱导先于侧根诱导后的第五分裂周期的完成。在烟草原生质体中，仅当诱导细胞分裂时才观察到 cyc1At 表达增加。总之，结果表明拟南芥中 Cyc1At 的积累受到转录调控，可能是细胞分裂激活的限制因素之一。
In eukaryotes, the control of cell cycle progression is exercised by heteromeric protein kinase complexes composed of a cell cycle-dependent, kinase-related subunit (Cdc2) and a cyclin subunit. To explore the possibility that cyclin transcription plays a role in the developmental regulation of cell division, we examined the spatial and temporal expression of a cyclin gene (cyc1At) in Arabidopsis. In root and shoot apical meristems and during embryogenesis, cyc1At expression is almost exclusively confined to dividing cells. A cell-specific pattern of cyc1At expression was noticed in root meristems. We examined the effects of induction of cell division of differentiated cells on cyc1At expression. During lateral root formation, induction of cyc1At expression is a very early event and was detected before anatomical modifications were visible. Treatment of roots with oryzalin, which blocks cell division in metaphase, did not inhibit the auxin induction of cyc1At, suggesting that induction of cyc1At expression precedes the completion of the fi mt division cycle af ter induction of lateral roots. In tobacco protoplasts, an increase in cyc1At expression was observed only when cell division was induced. Together, the results suggest that Cyc1At accumulation in Arabidopsis is transcriptionally regulated and might be one of the limiting factors for the activation of cell division.