Optical probing and imaging of live cells using SERS labels
Optical probing and imaging of live cells using SERS labels
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DOI：
10.1002/jrs.2060
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发表时间：
2009-01-01
影响因子：
2.5
通讯作者：
Kneipp, Katrin
中科院分区：
文献类型：
--
作者：
Kneipp, Janina;Kneipp, Harald;Kneipp, Katrin
在表面增强拉曼散射 (SERS) 过程中，当分子附着或非常靠近金或银纳米结构时，其拉曼信号会显着增加。这种效应被用作新型光学标签的基础。在这里，我们展示了稳健且灵敏的 SERS 标记作为活细胞成像探针。这些混合标签由金纳米颗粒组成，并附有玫瑰红或水晶紫作为报告分子。这些新标记稳定且无毒，不会发生光漂白，并且可以在任何激发波长下被激发，甚至在近红外区域。 SERS 标签可以通过记者的特定拉曼签名进行检测和成像。此外，金纳米颗粒局部光场中的表面增强拉曼光谱还提供了有关细胞中标记的直接分子环境的敏感信息，并允许对细胞的天然成分进行成像。基于报告器特征拉曼线的图像以及基于类似于 1470 cm(-1) 的 C-H 变形/弯曲模式的 SERS 信号显示脂质都证明了这一点。版权所有 (c) 2008 John Wiley & Sons, Ltd.
During surface-enhanced Raman scattering (SERS), molecules exhibit a significant increase in their Raman signals when attached, or in very close vicinity, to gold or silver nanostructures. This effect is exploited as the basis of a new class of optical labels. Here we demonstrate robust and sensitive SERS labels as probes for imaging live cells. These hybrid labels consist of gold nanoparticles with Rose Bengal or Crystal Violet attached as reporter molecules. These new labels are stable and nontoxic, do not suffer from photobleaching, and can be excited at any excitation wavelength, even in the near infrared. SERS labels can be detected and imaged through the specific Raman signatures of the reporters. In addition, surface-enhanced Raman spectroscopy in the local optical fields of the gold nanoparticles also provides sensitive information on the immediate molecular environment of the label in the cell and allows imaging of the native constituents of the cell. This is demonstrated by images based on a characteristic Raman line of the reporter as well as by displaying lipids based on the SERS signal of the C-H deformation/bending modes at similar to 1470 cm(-1). Copyright (c) 2008 John Wiley & Sons, Ltd.