Rhodococcus erythropolis as the receptor of cell-based sensor for 2,4-dinitrophenol detection:: effect of 'co-oxidation'
Rhodococcus erythropolis as the receptor of cell-based sensor for 2,4-dinitrophenol detection:: effect of 'co-oxidation'
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DOI：
10.1016/s0032-9592(01)00257-6
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发表时间：
2002-02-01
影响因子：
4.4
通讯作者：
Reshetilov, AN
中科院分区：
文献类型：
--
作者：
Emelyanova, EV;Reshetilov, AN
采用氧克拉克型电极作为传感器，用红红红球菌的全细胞作为微生物传感器的受体，测定2,4- dnp。在2 × 10(-5) ~ 20 × 10(-5) M的浓度范围内，传感器响应（作为受体的红毛鼠的呼吸速率）与2,4- dnp浓度呈线性关系。传感器对2,4- dnp的响应由两部分组成：(1)2,4- dnp作为呼吸底物对受体元件细胞呼吸的影响；(2) 2,4- dnp作为质子载体对细胞呼吸的促进作用。利用2.4-DNP在乙醇作为附加外源底物存在时对细胞呼吸的影响（共氧化效应），可以实现浓度低于2 x 10 M的二硝基苯酚检测。讨论了“共氧化效应”的机理。结果表明，红杉对乙醇的呼吸活性与2.4-DNP的呼吸活性之间存在一定的关系。这种活性的水平被认为取决于细胞中烟酰胺核苷酸的含量和这些核苷酸的氧化还原状态。(C) 2002 Elsevier Science Ltd.版权所有。
Whole cells of Rhodococcus erythropolis were employed as a receptor of a microbial sensor for 2,4-DNP determination using an oxygen Clark-type electrode as a transducer. A linear relationship of the sensor response (the respiratory rate of R. erythropolis as receptor) to 2,4-DNP concentrations was obtained for concentrations in the range from 2 x 10(-5) to 20 x 10(-5) M. Dinitrophenol concentrations of above 10(-3) M inhibited R. erythropolis respiration. The sensor response to 2,4-DNP was shown to add up from two components: (1) the effect of 2,4-DNP as the respiratory substrate on the cells' respiration of receptor element; and (2) the stimulative effect of 2,4-DNP as protonophore on the cells' respiration.Dinitrophenol detection at concentrations of below 2 x 10 M can be realized using the influence of 2.4-DNP upon the cells respiration in the presence of ethanol as an additional exogenous substrate (co-oxidation effect'). The mechanism of the 'co-oxidation effect' is discussed. A relationship between the activity of R. erythropolis respiration on ethanol and the activity of respiration on 2.4-DNP was found to exist. The level of this activity is supposed to depend on the content of nicotinamide nucleotides in the cells and oxidation-reduction state of these nucleotides. (C) 2002 Elsevier Science Ltd. All rights reserved.